文献类型：期刊文献

英文题名：Hsf and Hsp gene families in Populus: genome-wide identification, organization and correlated expression during development and in stress responses

作者：Zhang, Jin[1,2] Liu, Bobin[1,3] Li, Jianbo[1] Zhang, Li[1] Wang, Yan[4] Zheng, Huanquan[5] Lu, Mengzhu[1,2] Chen, Jun[1]

第一作者：Zhang, Jin

通信作者：Lu, MZ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat State Forestry A, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China;[3]Fujian Agr & Forestry Univ, Coll Forestry, Fuzhou 350002, Fujian, Peoples R China;[4]Shandong Agr Univ, Coll Life Sci, Shandong Key Lab Crop Biol, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China;[5]McGill Univ, Dept Biol, Montreal, PQ H3A 1B1, Canada

年份：2015

卷号：16

期号：1

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000351502400001)】；

基金：This work was supported by the National High Technology Research and Development Program of China [2013AA102702] and the National Natural Science Foundation of China [31270699] to JC, the National Key Basic Research Program of China [2012CB114500] and a Collaborative Innovation Plan of Jiangsu Higher Education to ML, the China Postdoctoral Science Foundation [2014 M550104] to JZ, and the lecture and study program for outstanding scholars from home and abroad (CAFYBB2011007).

语种：英文

外文关键词：Coexpression; Expression analysis; Gene family; Heat shock factor (Hsf); Heat shock protein (Hsp); Populus

摘要：Background: Heat shock proteins ( Hsps) are molecular chaperones that are involved in many normal cellular processes and stress responses, and heat shock factors (Hsfs) are the transcriptional activators of Hsps. Hsfs and Hsps are widely coordinated in various biological processes. Although the roles of Hsfs and Hsps in stress responses have been well characterized in Arabidopsis, their roles in perennial woody species undergoing various environmental stresses remain unclear. Results: Here, a comprehensive identification and analysis of Hsf and Hsp families in poplars is presented. In Populus trichocarpa, we identified 42 paralogous pairs, 66.7% resulting from a whole genome duplication. The gene structure and motif composition are relatively conserved in each subfamily. Microarray and quantitative real-time RT-PCR analyses showed that most of the Populus Hsf and Hsp genes are differentially expressed upon exposure to various stresses. A coexpression network between Populus Hsf and Hsp genes was generated based on their expression. Coordinated relationships were validated by transient overexpression and subsequent qPCR analyses. Conclusions: The comprehensive analysis indicates that different sets of PtHsps are downstream of particular PtHsfs and provides a basis for functional studies aimed at revealing the roles of these families in poplar development and stress responses.