文献类型：期刊文献

英文题名：Integrated transcriptome and proteome analysis reveals brassinosteroid-mediated regulation of cambium initiation and patterning in woody stem.

作者：Wang, Congpeng[1,4] Liu, Naixu[1] Geng, Zhao[1] Ji, Meijing[1] Wang, Shumin[2] Zhuang, Yamei[2] Wang, Dian[3] He, Guo[2] Zhao, Shutang[5] Zhou, Gongke[1,4] Chai, Guohua[1,4]

第一作者：Wang, Congpeng

通信作者：Zhou, GK[1];Chai, GH[1];Zhou, GK[2];Chai, GH[2]

机构：[1]Qingdao Agr Univ, Coll Resources & Environm, Qingdao 266109, Peoples R China;[2]Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266101, Peoples R China;[3]Qingdao Agr Univ, Coll Agron, Qingdao 266109, Peoples R China;[4]Qingdao Agr Univ, Acad Dongying Efficient Agr Technol & Ind Saline, Dongying 257000, Peoples R China;[5]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2022

卷号：9

外文期刊名：HORTICULTURE RESEARCH

收录：;Scopus(收录号：2-s2.0-85134956576);WOS:【SCI-EXPANDED(收录号:WOS:000791049400046)】；

基金：We thank Prof. Yaoguang Liu (South China Agricultural University, China) for providing the CRISPR-Cas9 system and Prof. Wenqiang Tang (Heibei Normal University, China) for providing the Arabidopsis bri1 brl1 brl3 mutant seeds. Financial support for this work was obtained from the National Key Scientific Research Project of China (2021YFD2200205), the National Natural Science Foundation of China (31670606, 31972955, 31770315, and 31700526), the Taishan Scholar Program of Shandong (to GC and to GZ), and the "First-Class Grassland Science Discipline" program of Shandong Province.

语种：英文

摘要：Wood formation involves sequential developmental events requiring the coordination of multiple hormones. Brassinosteroids (BRs) play a key role in wood development, but little is known about the cellular and molecular processes that underlie wood formation in tree species. Here, we generated transgenic poplar lines with edited PdBRI1 genes, which are orthologs of Arabidopsis vascular-enriched BR receptors, and showed how inhibition of BR signaling influences wood development at the mRNA and/or proteome level. Six Populus PdBRI1 genes formed three gene pairs, each of which was highly expressed in basal stems. Simultaneous mutation of PdBRI1-1, -2, -3 and - 6, which are orthologs of the Arabidopsis vascular-enriched BR receptors BRI1, BRL1 and BRL3, resulted in severe growth defects. In particular, the stems of these mutant lines displayed a discontinuous cambial ring and patterning defects in derived secondary vascular tissues. Abnormal cambial formation within the cortical parenchyma was also observed in the stems of pdbri1-1;2;3;6. Transgenic poplar plants expressing edited versions of PdBRI1-1 or PdBRI1-1;2;6 exhibited phenotypic alterations in stem development at 4.5 months of growth, indicating that there is functional redundancy among these PdBRI1 genes. Integrated analysis of the transcriptome and proteome of pdbri1-1;2;3;6 stems revealed differential expression of a number of genes/proteins associated with wood development and hormones. Concordant (16%) and discordant (84%) regulation of mRNA and protein expression, including wood-associated mRNA/protein expression, was found in pdbri1-1;2;3;6 stems. This study found a dual role of BRs in procambial cell division and xylem differentiation and provides insights into the multiple layers of gene regulation that contribute to wood formation in Populus.