文献类型：期刊文献

中文题名：9种瘿蚜的Wolbachia分子检测和系统发育分析

英文题名：Molecular Detection and Phylogenetic Analysis of Wolbachia in Nine Gall Aphid Species

作者：任维宾[1] 杨子祥[1] 杨瑛[1] 吴海霞[1] 陈航[1]

第一作者：任维宾

机构：[1]中国林业科学研究院资源昆虫研究所国家林业局资源昆虫培育与利用重点实验室

年份：2018

卷号：31

期号：3

起止页码：1-8

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：中央级公益性科研院所基本科研业务费专项(CAFYBB2014ZD005;riricaf2014001Z);国家自然科学基金(U1402263;31370651;31372266)

语种：中文

中文关键词：瘿蚜;次级共生菌;沃尔巴克氏体;系统发育分析;超群

外文关键词：gall aphid;facuhative symbiont;Wolbachia;phylogenetic analysis;supergroup

分类号：S899.4

摘要：[目的]以五倍子蚜、杨树瘿蚜和黄连木瘿蚜等9种瘿蚜为实验材料,探讨瘿蚜Wolbachia与其它自由生活蚜虫携带的Wolbachia的株系差异及亲缘关系,明确五倍子蚜、杨树瘿蚜和黄连木瘿蚜等9种瘿蚜的Wolbachia感染情况、株系及系统发育关系。[方法]从田间采集新鲜虫瘿,将蚜虫转移到离心管内,100%乙醇-20℃保存。采用Wolbachia的16S rRNA、wsp、fts Z、gro E和glt A基因引物,以瘿蚜的总DNA为模板进行扩增、测序和分析,联合NCBI中Wolbachia 16S rRNA相关基因序列进行系统发育分析。[结果]在检测的9种瘿蚜中,有3种瘿蚜共3个种群感染有Wolbachia,其中滇叶瘿绵蚜昆明种群的Wolbachia属于B超群,肚倍蚜昆明种群和角倍蚜峨眉种群的Wolbachia可能属于新发现的O超群。[结论]与自由生活的蚜虫相比,瘿蚜的Wolbachia感染率偏低,且株系间的差异较大。
[Objective]To determine the infection and phylogenetic relationships of Wolbachia in nine gall aphids hosted on Rhus spp.,Populus yunnanensis and Pistacia chinensis. [Method]Fresh aphid galls were collected in the field and transferred the aphids to an Eppendorf tube,and placed in 100% ethanol then deposited in-20℃. The genomic DNA was amplified and sequenced using the primers of 16 S rRNA,wsp,fts Z,gro E and glt A genes. The phylogenetic relationships were analysis using Wolbachia 16 S rRNA gene sequences and relevant gene sequences from NCBI. [Result]Three species among the nine gall aphids species were infected with Wolbachia. The strain from Pemphigus yunnanensis of Kunming population belongs to B supergroup,and strain from Kaburagia rhusicola of Kunming population and Schlechtendalia chinensis of Emei population belong to O supergroup. [Conclusion]Compared with the free-living aphids,Wolbachia infected in gall aphids have low infection rates and significant differences among strains.