文献类型：期刊文献

英文题名：Genome-wide analysis of the TPX2 family proteins in Eucalyptus grandis

作者：Du, Pingzhou[2,3,4,5] Kumar, Manoj[6] Yao, Yuan[2,3,4] Xie, Qiaoli[3,4] Wang, Jinyan[5] Zhang, Baolong[5] Gan, Siming[1] Wang, Yuqi[2,7] Wu, Ai-Min[3,4]

第一作者：Du, Pingzhou

通信作者：Gan, SM[1];Wang, YQ[2];Wu, AM[3];Wu, AM[4];Wang, YQ[5]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[2]South China Agr Univ, Coll Life Sci, Guangzhou 510642, Guangdong, Peoples R China;[3]South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Guangdong, Peoples R China;[4]South China Agr Univ, Coll Forestry & Landscape Architecture, Guangdong Key Lab Innovat Dev & Utilizat Forest P, Guangzhou 510642, Guangdong, Peoples R China;[5]Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Nanjing 210014, Jiangsu, Peoples R China;[6]Univ Manchester, Fac Life Sci, Michael Smith Bldg,Oxford Rd, Manchester M13 9PT, Lancs, England;[7]Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA

年份：2016

卷号：17

期号：1

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000388773300003)】；

基金：This work was supported by Ministry of Science and Technology of China (Grant Number 2013AA102705), National Natural Science Foundation of China (Grant Number 31270594), open fund of state key laboratory of tree genetics and breeding (Grant Number TGB2015007) and Guangzhou science and technology plan projects (Grant Number 201300000200).

语种：英文

外文关键词：Eucalyptus; TPX2 family proteins; Phylogenetic analysis; Expression profiling; Subcellular localization; Organ twisting

摘要：Background: The Xklp2 (TPX2) proteins belong to the microtubule-associated (MAP) family of proteins. All members of the family contain the conserved TPX2 motif, which can interact with microtubules, regulate microtubule dynamics or assist with different microtubule functions, for example, maintenance of cell morphology or regulation of cell growth and development. However, the role of members of the TPX family have not been studied in the model tree species Eucalyptus to date. Here, we report the identification of the members of the TPX2 family in Eucalyptus grandis (Eg) and analyse the expression patterns and functions of these genes. Results: In present study, a comprehensive analysis of the plant TPX2 family proteins was performed. Phylogenetic analyses indicated that the genes can be classified into 6 distinct subfamilies. A genome-wide survey identified 12 members of the TPX2 family in the sequenced genome of Eucalyptus grandis. The basic genetic properties of the TPX2 family in Eucalyptus were analysed. Our results suggest that the TPX2 family proteins within different sub-groups are relatively conserved but there are important differences between groups. Quantitative real-time PCR (qRT-PCR) was performed to confirm the expression levels of the genes in different tissues. The results showed that in the whole plant, the levels of EgWDL5 transcript are the highest, followed by those of EgWDL4. Compared with other tissues, the level of the EgMAP20 transcript is the highest in the root. Over-expression of EgMAP20 in Arabidopsis resulted in organ twisting. The cotyledon petioles showed left-handed twisting while the hypocotyl epidermal cells produced right-handed helical twisting. Finally, EgMAP20, EgWDL3 and EgWDL3L were all able to decorate microtubules. Conclusions: Plant TPX2 family proteins were systematically analysed using bioinformatics methods. There are 12 TPX2 family proteins in Eucalyptus. We have performed an initial characterization of the functions of several members of the TPX2 family. We found that the gene products are localized to the microtubule cytoskeleton. Our results lay the foundation for future efforts to reveal the biological significance of TPX2 family proteins in Eucalyptus.