文献类型：期刊文献

英文题名：PagEXPA1 combines with PagCDKB2;1 to regulate plant growth and the elongation of fibers in Populus alba x Populus glandulosa

作者：Hao, Yuanyuan[1] Chu, Liwei[1,3] He, Xuejiao[1] Zhao, Shutang[1,2] Tang, Fang[1,2,4]

第一作者：Hao, Yuanyuan

通信作者：Tang, F[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm,Res Inst Forestry, Beijing, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China;[3]Dalian Univ, Coll Life Hlth, Dalian 116622, Liaoning, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2024

卷号：268

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001235438900001)】；

基金：This work was supported by The Science and Technology Innovation 2030 (2023ZD04062) and the National Natural Science Foundation of China (31700592) .

语种：英文

外文关键词：Populus; Transgenic plants; Fiber elongation; Plants development; EXPA1; CDKB2

摘要：Expansins are important plant cell wall proteins. They can loosen and soften the cell walls and lead to wall extension and cell expansion. To investigate their role in wood formation and fiber elongation, the PagEXPA1 that highly expressed in cell differentiation and expansion tissues was cloned from 84K poplar ( Populus alba x P. glandulosa ). The subcellular localization showed that PagEXPA1 located in the cell wall and it was highly expressed in primary stems and young leaves. Compared with non-transgenic 84K poplar, overexpression of PagEXPA1 can promote plant-growth, lignification, and fiber cell elongation, while PagEXPA1 Cas9-editing mutant lines exhibited the opposite phenotype. Transcriptome analysis revealed that DEGs were mainly enriched in some important processes, which are associated with cell wall formation and cellulose synthesis. The protein interaction prediction and expression analysis showed that PagCDKB2:1 and PagEXPA1 might have an interaction relationship. The luciferase complementary assay and bimolecular fluorescence complementary assay validated that PagEXPA1 can combined with PagCDKB2;1. So they promoted the expansion of xylem vascular tissues and the development of poplar though participating in the regulation of cell division and differentiation by programming the cell-cycle. It provides good foundation for molecular breeding of fast -growing and highquality poplar varieties.