文献类型：期刊文献

英文题名：Over-expression of the Arabidopsis Na+/H+ antiporter gene in Populus deltoides CL x P. euramericana CL "NL895" enhances its salt tolerance

作者：Qiao, Guirong[1] Zhuo, Renying[1] Liu, Mingying[1] Jiang, Jing[1] Li, Haiying[1] Qiu, Wenmin[1] Pan, Luanyin[1] Lin, Sheng[1,3] Zhang, Xiaoguo[1] Sun, Zongxiu[2]

第一作者：乔桂荣

通信作者：Zhuo, RY[1]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Genom, Fuyang 311400, Zhejiang, Peoples R China;[2]China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China;[3]Beijing Univ Chem Technol, Beijing 100029, Peoples R China

年份：2011

卷号：33

期号：3

起止页码：691-696

外文期刊名：ACTA PHYSIOLOGIAE PLANTARUM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000289476300006)】；

基金：The authors thank Dr. Roberto A. Gaxiola at the University of Connecticut for kindly providing AtNHX1 cDNA. This work was supported by the National Natural Science Foundation of China (30972340) and Zhejiang Provincial Natural Science Foundation of China under Grant No. R3090070.

语种：英文

外文关键词：AtNHX1; Populous; Mannose selection system; Salt stress

摘要：Soil salinity is a serious problem worldwide. It is necessary to improve the salt tolerance of plants to avoid the progressive deterioration of saline soil. We showed that the over-expression of AtNHX1 improves salt tolerance in a transgenic poplar (Populus deltoides CL x P. euramericana CL "NL895") under mannose selection. Four transgenic poplar plants were obtained. Southern blot analysis showed that the pmi gene had integrated into the genome of the poplar. RT-PCR confirmed that AtNHX1 could be expressed normally in the transgenic plants. When tested for salt tolerance by NaCl stress, we measured a 100% increase in Na+ content in the three transgenic lines (T18, T50, T98) significantly higher than the 33% increase seen in wild-type plants. The chlorophyll content of the transgenic plants was not altered significantly, while the chlorophyll content in the control plants showed a small decrease. MDA content was decreased in the transgenic plants. These results show that the AtNHX1 gene may enhance salt tolerance due to increased vacuolar compartmentalization of sodium ions.