文献类型：期刊文献

英文题名：Optimized GC-MS Method To Simultaneously Quantify Acetylated Aldose, Ketose, and Alditol for Plant Tissues Based on Derivatization in a Methyl Sulfoxide/1-Methylimidazole System

作者：Li, Ke[1,3] Liu, Shichang[1] Tan, Yupeng[1] Chao, Nan[1] Tian, Xiaoming[1] Qi, Liwang[4] Powell, William A.[5] Jiang, Xiangning[1,2] Gai, Ying[1,2]

第一作者：Li, Ke

通信作者：Gai, Y[1]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China;[2]State Forestry Adm, Tree & Ornamental Plant Breeding & Biotechnol Lab, Beijing 100083, Peoples R China;[3]Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Taiyuan 030006, Shanxi, Peoples R China;[4]Chinese Acad Forestry, Inst Forestry Res, Beijing 100091, Peoples R China;[5]SUNY Coll Environm Sci & Forestry, Syracuse, NY 13205 USA

年份：2013

卷号：61

期号：17

起止页码：4011-4018

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20131916318329);Scopus(收录号：2-s2.0-84877029071);WOS:【SCI-EXPANDED(收录号:WOS:000318468900003)】；

基金：This work was jointly supported by the 12th-Five Year Development Project ("863" 2011AA100203), a National Natural Science Foundation Project (Grant 30630053), and a BJFU New Scientist Initiate Project (2010BLX03).

语种：英文

外文关键词：aldose; ketose; methyl sulfoxide; acetic anhydride; 1-methylimidazole; GC-MS

摘要：The isomers of monosaccharide always produce multiple chromatographic peaks as volatile derivatives during gas chromatography, which may result in the overlapping of different sugar peaks. Whereas reduction and oximation of sugar carbonyl groups for GC analysis do eliminate many isomer derivatives, the approaches create new problems. One ketose can yield two peaks by oximation, and different aldoses and ketoses can yield the same alditol upon reduction, leading to the inability to detect some important monosaccharides. This paper reports an optimal method that yields a single peak per sugar by acetylation directly. By using a methyl,sulfoxide (Me2SO)/1-methylimidazole (1-MeIm) system; the carbohydrates in acetic anhydride (Ac2O) esterification reactions were solubilized, and the oxidation that normally occurs was inhibited. The results demonstrate that acetylated derivatives f 23 saccharides had unique peaks, which indicates aldose, ketose, and alditol can be determined simultaneously by GC-MS.