文献类型：期刊文献

中文题名：圣音竹及其回复突变体笋期的碳氮代谢特征分析

英文题名：Physiological features of C and N metabolism during shoot elongation in Phyllostachys edulis f.tubaeformis and its reverse mutant

作者：岳晋军[1,2] 王涛[3] 彭镇华[1,3] 李潞滨[1,3] 顾小平[2] 袁金玲[2] 吴晓丽[2] 刘正娥[2]

第一作者：岳晋军

机构：[1]国际竹藤中心;[2]中国林业科学研究院亚热带林业研究所;[3]中国林业科学研究院林业研究所

年份：2018

卷号：42

期号：1

起止页码：60-66

中文期刊名：南京林业大学学报：自然科学版

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：浙江省林木新品种选育重大科技专项(2016C02056-8);浙江省公益技术研究农业项目(2015C32012);中央级公益性科研院所基本科研业务费专项资金项目(RISF2013004)

语种：中文

中文关键词：圣音竹;秆型变异;生理指标;碳氮代谢;回复突变体

外文关键词：Phyllostachys edulis f. tubaeformis; culm variation ; physiological feature; C and N metabolism; reverse mutant

分类号：S722

摘要：【目的】碳氮代谢是竹子节间伸长的重要生理活动,研究节间短缩的圣音竹及节长正常的回复突变体两者在碳氮代谢上的差异,从而揭示影响竹材利用率的主要性状——节间长度在代谢层面的调控机制。【方法】以圣音竹及其回复突变体节间快速伸长时期的竹笋为材料,对其笋体和笋箨不同部位的有关碳氮代谢主要物质及相关酶活性进行了测定。【结果】在笋体部位,圣音竹的蔗糖和果糖含量,以及酸性蔗糖转化酶活性显著低于其回复突变体,中性蔗糖转化酶活性则显著高于后者,而葡萄糖含量在两者间差别不大;圣音竹的蛋白质含量、谷氨酸脱氢酶活性显著低于其回复突变体,铵态氮含量显著大于后者,硝态氮含量在两者间相差不大。在笋箨部位,多数指标差异不显著。【结论】从碳氮代谢的角度看,圣音竹笋体中蔗糖浓度降低和氮代谢异常可能是导致其节间短缩的原因之一。
【Objective】Internodal length is an important phenotypic parameter affecting the usage of bamboo timber in order to explore the mechanism of its growth.【Method】The physiological features of carbohydrate and nitrogen metabolism in shoots and sheaths were analyzed using Phyllostachys edulis f.tubaeformis and its reverse mutant as subjects of the study.【Result】The contents of sucrose,fructose and acid invertase activity were significantly lower,while neutral invertase activity was significantly higher in the shoots of Ph.edulis f.tubaeformis than that in its reverse mutant,and there was no significant difference in glucose content.Protein content and glutamate dehydrogenase activity were significantly lower,while ammonium nutrient content was significantly higher in the shoots of Ph.edulis f.tubaeformis than that in its reverse mutant,and there was no significant difference in nitrate nitrogen content.These characteristics of carbohydrate and nitrogen metabolism demonstrated no significant difference in sheaths.【Conclusion】Based on these results,the decrease in sucrose content and abnormality in nitrogen metabolism in the shoots were supposed to be reasons for internode shortening in Ph.edulis f.tubaeformis.This study investigated for the first time in physiological mechanism of internode shortening in Ph.edulis f.tubaeformis,thus providing a basis for further genomic studies.