文献类型：期刊文献

英文题名：CAD1 and CCR2 protein complex formation in monolignol biosynthesis in Populus trichocarpa

作者：Yan, Xiaojing[1,2] Liu, Jie[3] Kim, Hoon[4,5] Liu, Baoguang[6,7] Huang, Xiong[1] Yang, Zhichang[8] Lin, Ying-Chung Jimmy[6,9] Chen, Hao[3] Yang, Chenmin[3] Wang, Jack P.[3,6] Muddiman, David C.[8] Ralph, John[4,5] Sederoff, Ronald R.[3] Li, Quanzi[1,2] Chiang, Vincent L.[3,6]

第一作者：闫晓婧

通信作者：Li, QZ[1];Li, QZ[2];Chiang, VL[3];Chiang, VL[4]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[3]North Carolina State Univ, Dept Forestry & Environm Resources, Forest Biotechnol Grp, Raleigh, NC 27695 USA;[4]Univ Wisconsin, Wisconsin Energy Inst, Dept Biochem, Madison, WI 53726 USA;[5]Univ Wisconsin, Wisconsin Energy Inst, DOE Great Lakes Bioenergy Res Ctr, Madison, WI 53726 USA;[6]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Heilongjiang, Peoples R China;[7]Beihua Univ, Dept Forestry, Jilin 132013, Jilin, Peoples R China;[8]North Carolina State Univ, Dept Chem, WM Keck FT ICR Mass Spectrometry Lab, Raleigh, NC 27695 USA;[9]Natl Taiwan Univ, Inst Plant Biol, Dept Life Sci, Coll Life Sci, Taipei 10617, Taiwan

年份：2019

卷号：222

期号：1

起止页码：244-260

外文期刊名：NEW PHYTOLOGIST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000459928400025)】；

基金：This work was supported by grants from the Fundamental Research Funds of CAF (CAFYBB2016ZX001-1), the National Key Research and Development Program of China (2016YFD0600103) and the National Science Foundation Plant Genome Research Program Grant (DBI-0922391). HK and JR (and the NMR instrumentation used in this work) were partially funded by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494 and DE-SC0018409).

语种：英文

外文关键词：co-immunoprecipitation; enzyme activity; monolignol biosynthetic pathway; nuclear magnetic resonance (NMR); Populus trichocarpa; stem-differentiating xylem protein

摘要：Lignin is the major phenolic polymer in plant secondary cell walls and is polymerized from monomeric subunits, the monolignols. Eleven enzyme families are implicated in monolignol biosynthesis. Here, we studied the functions of members of the cinnamyl alcohol dehydrogenase (CAD) and cinnamoyl-CoA reductase (CCR) families in wood formation in Populus trichocarpa, including the regulatory effects of their transcripts and protein activities on monolignol biosynthesis. Enzyme activity assays from stem-differentiating xylem (SDX) proteins showed that RNAi suppression of PtrCAD1 in P. trichocarpa transgenics caused a reduction in SDX CCR activity. RNAi suppression of PtrCCR2, the only CCR member highly expressed in SDX, caused a reciprocal reduction in SDX protein CAD activities. The enzyme assays of mixed and coexpressed recombinant proteins supported physical interactions between PtrCAD1 and PtrCCR2. Biomolecular fluorescence complementation and pull-down/co-immunoprecipitation experiments supported a hypothesis of PtrCAD1/PtrCCR2 heterodimer formation. These results provide evidence for the formation of PtrCAD1/PtrCCR2 protein complexes in monolignol biosynthesis in planta.