文献类型：期刊文献

英文题名：Genomic survey and expression analysis of the CDPK genes in Liriodendron Chinense to explore their potential functions under multiple abiotic stresses

作者：Guan, Yuanlin[1,2] Liu, Siqin[3] Hwarari, Delight[2] Wang, Yunpeng[4] Yang, Liming[2] Yao, Jiabao[1]

第一作者：Guan, Yuanlin

通信作者：Yao, JB[1]

机构：[1]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Biodivers Conservat & Resource Utilizat Jiangxi Pr, Fenyi, Peoples R China;[2]Nanjing Forestry Univ, Coll Life Sci, State Key Lab Tree Genet & Breeding, Nanjing, Peoples R China;[3]Chinese Acad Forestry, Res Inst Nontimber Forestry, Zhengzhou, Peoples R China;[4]Jiangxi Acad Sci, Inst Biol Resources, Jiangxi Prov Key Lab Improved Variety Breeding & E, Nanchang, Jiangxi, Peoples R China

年份：2026

卷号：27

期号：1

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001691401900001)】；

基金：This research work was supported by the Key Research and Development Program of Jiangxi Province(20252BCF320009), and the Jiangxi Provincial Natural Science Foundation(20232BAB205047).

语种：英文

外文关键词：Liriodendron chinense; CDPK genes; Subcellular localization; Abiotic stress; Expression analysis

摘要：Background Calcium-dependent protein kinases (CDPKs) are key components of calcium signal transduction pathway, which play crucial roles in plant growth, development and abiotic stress response. The forest tree Liriodendron chinense possesses high ecological, ornamental, and economic value; however, abiotic stresses restrict its growth, reproductive capacity, and geographical distribution. The identification of the CDPK gene family in L. chinense, along with analyses of their structural and evolutionary relationships, tissue-specific expression patterns, will facilitate the elucidation of their functions in responding to abiotic stresses. Results A total of 21 CDPK genes were identified in the L. chinense genome and phylogenetically classified into four distinct groups. Amino acid sequences of most CDPKs contained four typical EF-hand structures.The cis-acting elements analysis of the promoter region showed a general representation of the ARE, MBS and LTR elements related to abiotic stress. RNA-seq analysis revealed that 6 h, 24 h, and 3 d post-abiotic stress were critical time points for the plant's regulatory responses. RT-qPCR expression profiles demonstrated that the selected LcCDPKs exhibited differential expression across various tissues, and expression profiling suggested a collective trend of responsiveness to these stresses, with the most pronounced and sustained transcriptional changes often observed under heat stress, followed by cold and then drought. Subcellular localization assays indicated that LcCDPK5-GFP and LcCDPK10-GFP were localized to the nucleus and cell membrane, while LcCDPK5 was additionally localized to the cytoplasm. Conclusions This study systematically identified CDPKs in L. chinense, and indicates their potential roles in response to abiotic stress.This study provides important insights into the evolution and function of CDPKs in L. chinense.