文献类型：期刊文献

英文题名：Stage-specific regulation of four HD-ZIP III transcription factors during polar pattern formation in Larix leptolepis somatic embryos

作者：Li, Shui-gen[1] Li, Wan-feng[1] Han, Su-ying[2] Yang, Wen-hua[1] Qi, Li-wang[1]

第一作者：Li, Shui-gen

通信作者：Qi, LW[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Cell Biol Lab, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Key Lab Res Inst Forest Ecol & Protect, Beijing 100091, Peoples R China

年份：2013

卷号：522

期号：2

起止页码：177-183

外文期刊名：GENE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000320147000007)】；

基金：This work was supported by the National Basic Research Program of China (2009CB119100), the National High Technology Research and Development Program of China (2011AA100203) and the National Natural Science Foundation of China (30830086).

语种：英文

外文关键词：Somatic embryo; HD-ZIP III; Polar auxin transport; NPA; Larix leptolepis

摘要：Polar auxin transport provides a developmental signal for cell fate specification during somatic embryogenesis. Some members of the HD-ZIP III transcription factors participate in regulation of auxin transport, but little is known about this regulation in somatic embryogenesis. Here, four HD-ZIP III homologues from Larix leptolepis were identified and designated LaHDZ31, 32,33 and 34. The occurrence of a miR165/166 target sequence in all four cDNA sequences indicated that they might be targets of miR165/166. Identification of the cleavage products of LaHDZ31 and LaHDZ32 in vivo confirmed that they were regulated by miRNA. Their mRNA accumulation patterns during somatic embryogenesis and the effects of 1-N-naphthylphthalamic acid (NPA) on their transcript levels and somatic embryo maturation were investigated. The results showed that the four genes had higher transcript levels at mature stages than at the proliferation stage, and that NPA treatment down-regulated the mRNA abundance of LaHDZ31, 32 and 33 at cotyledonary embryo stages, but had no effect on the mRNA abundance of LaHDZ34. We concluded that these four members of Larix HD-ZIP III family might participate in polar auxin transport and the development of somatic embryos, providing new insights into the regulatory mechanisms of somatic embryogenesis. (C) 2013 Elsevier B.V. All rights reserved.