文献类型：期刊文献

英文题名：PdMATE16 and PdMATE35 are essential for the accumulation of multiple flavonoids in tree peonies

作者：Yuan, Meng[1] Wei, Xinrui[1] Peng, Fucheng[1] Wang, Qun[1] Zhou, Lin[1] Wang, Yan[1]

第一作者：Yuan, Meng

通信作者：Zhou, L[1];Wang, Y[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm, Beijing, Peoples R China

年份：2025

卷号：16

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-105023895634);WOS:【SCI-EXPANDED(收录号:WOS:001629379000001)】；

基金：The author(s) declare financial support was received for the research and/or publication of this article. This research was supported by the National Key Research and Development Program of China (Grant No. 2018YFD1000405) and the National Natural Science Foundation of China (Grant No. 31972456).

语种：英文

外文关键词：mate; gene family; transport; flavonoid; Paeonia delavayi var. lutea; flower color

摘要：As a plant with both medicinal and edible uses, peonies are not only valued for their ornamental qualities, but also for the high economic value of the flavonoids found in their petals. However, the regulatory mechanisms governing flower color and flavonoid transport remain unclear. The multidrug and toxic compound extrusion (MATE) family of proteins are important transport protein involved in the transport of a variety of secondary metabolites in plants. Based on transcriptomic data, this study identified 37 MATE genes from the transcriptomic data of Paeonia delavayi var. lutea. Molecular docking and site-directed mutagenesis results predict that PdMATE16 and PdMATE35 can bind multiple pigments. Overexpression and virus-induced gene silencing (VIGS) experiments further confirmed that PdMATE16 and PdMATE35 could transport a variety of flavonoid compounds, including flavone glycosides, flavonol glycosides, chalcone glycosides and anthocyanin glycosides. These substances not only give peonies their yellow color, but are also the main active ingredients in medicinal applications. In addition, several transcription factors that may be involved in regulating MATE gene expression were screened. Our research confirms the key role of PdMATE16 and PdMATE35 in flavonoid transport in peonies, clarifies the regulatory mechanism underlying the transport processes involved in peony coloration, and may thus contribute to the improvement of ornamental and medicinal plants.