文献类型：期刊文献

中文题名：西南桦花粉离体萌发的适宜条件与萌发特征

英文题名：Preliminary Report on in Vitro Pollen Germination of Betula alnoides

作者：程伟[1] 赵志刚[1] 曾杰[1] 郭俊杰[1] 赖家业[2] 温远光[2]

第一作者：程伟

机构：[1]中国林业科学研究院热带林业研究所;[2]广西大学林学院

年份：2007

卷号：20

期号：2

起止页码：209-212

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家"十一五"科技支撑专题"优质珍贵用材树种桦木;水曲柳新品种优育"(2006BAD01A1605)的子专题"优质珍贵用材树种西南桦新品种选育";国家自然科学基金项目(30400345)的部分内容

语种：中文

中文关键词：西南桦;花粉离体萌发;花粉管生长

外文关键词：Betula alnoides ;in vitro pollen germination;pollen tube growth

分类号：S722.3

摘要：应用各种浓度蔗糖、蔗糖+硼酸培养基于系列温度下开展西南桦花粉萌发对比试验,研究了蔗糖、硼酸浓度对西南桦花粉离体萌发的影响,筛选出适合西南桦花粉萌发培养基的适宜组分和适宜培养温度,并应用4个单株的花粉对其萌发特征进行了初步研究。结果表明:蔗糖基本培养基上添加硼酸能够显著促进西南桦花粉萌发;西南桦花粉离体萌发的合适培养基为15%蔗糖+200 mg.kg-1硼酸,适宜培养温度为30℃;在此条件下培养3 h后花粉萌发率基本稳定,培养7 h后花粉管长度也趋于稳定。
In this paper suitable germinating media and optimized temperature for pollens of Betala alnoides Buch. Ham. ex D. Don were screened out through comparison tests using all sorts of germinating media with different concentrations of sugar and boric acid under a series of temperature conditions, and its pollen germination process was characterized with pollens collected from four individuals. It was indicated that supplementing boric acid to sugar media could remarkably accelerate pollen germination of B. a/no/des, the suitable media for pollen germination consist of 15% sucrose and 200 mg. kg^-1 boric acid in distilled water, and optimized temperature was 30 ℃ for this species. And under these good conditions, the pollen germination rate and pollen tube length became stabilized after three hours and seven hours, respectively.