文献类型：期刊文献

中文题名：电导法和电阻抗图谱法测定脱锻炼期间欧美杨×大青杨F_1子代抗寒性

英文题名：Electrolyte Leakage and Electrical Impedance Spectroscopy Methods Assessing Frost Hardiness of F_1 Genotypes between Populus×canadensis Moench cv.'Neva'×Populus ussuriensis Kom.during Dehardening

作者：张洁[1] 张钢[1] 李金花[2] 张振英[1]

第一作者：张洁

机构：[1]河北农业大学园艺学院,河北保定071001;[2]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,北京100091

年份：2012

卷号：48

期号：1

起止页码：44-50

中文期刊名：植物生理学报

外文期刊名：Plant Physiology Journal

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：国家林业局“948”项目(2011-04-08);中国林业科学研究院林业研究所重点项目(ZD200905)

语种：中文

中文关键词：欧美杨×大青杨F_1子代;脱锻炼;抗寒性;胞外电阻率(re);弛豫时间(τ)

外文关键词：F1 hybrid between Populus×euranericana cv.＇Neva＇×P.ussuriensis Kom.; dehardening; frost hardiness; extracellular resistance（re）; relaxation time（τ）

分类号：S688.4

摘要：利用电导(EL)法和电阻抗图谱(EIS)法对脱锻炼期间欧美杨‘107杨’(Populus×canadensis Moench cv.‘Neva’)×大青杨(P.ussuriensis Kom.)F1子代进行抗寒性测定,试图通过比较两种方法测定抗寒性结果的相关性,以确定EIS法快速测定‘107杨’×大青杨F1子代抗寒性的最佳参数。以8个杂种子代和母本‘107杨’3年生为试材,进行了电阻抗图谱法和电导法分析,结果表明:脱锻炼期间,各子代抗寒性均逐渐降低,依次为NK36>NK41>NK61>NK57>NK42>NK60>NK58>NK56,均高于‘107杨’,EIS参数弛豫时间[EIS(τ)法]和EL法的抗寒性测定结果间存在明显的线性相关(R2=0.965),但EIS法测定的抗寒性低于EL法,且未经冷冻处理样本的胞外电阻率(re)和弛豫时间(τ)与经冷冻处理EL测定的抗寒性有较高的相关性,R2分别为0.817和0.847。
Frost hardiness（FH） of 8 genotypes from F1 hybrid between Populus×canadensis cv.＇Neva＇×Popu-lus ussuriensis Kom.during dehardening was assessed by methods of electrical impedance spectroscopy（EIS） and electrolyte leakage（EL）,and correlation between the results of two methods was compared in order to find the optimum EIS parameters for measuring the FH of the F1 genotypes.Three-year-old F1 genotypes and their female parent were sampled for EIS and EL analysis.The results indicated that：（1） FH of 8 F1 genotypes decreased during dehardening with a sequence of NK36NK41NK61NK57NK42NK60NK58NK56,which were superior to ＇Neva＇;（2） Linear correlation between the results of EIS（τ） and EL in assessing FH was significant（R2=0.965）.However,FH of EIS was underestimated than that of EL;（3） Without controlled freezing tests,extracellular resistance（re） and relaxation time（τ） of EIS were highly correlated with FH of EL（R2 were 0.817 and 0.847,respectively）.