文献类型：期刊文献

英文题名：Genome-wide analysis of long non-coding RNAs in Catalpa bungei and their potential function in floral transition using high-throughput sequencing

作者：Wang, Zhi[1] Zhu, Tianqing[1] Ma, Wenjun[1] Wang, Nan[1] Qu, Guanzheng[2] Zhang, Shougong[1] Wang, Junhui[1]

通信作者：Wang, JH[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, State Key Lab Tree Genet & Breeding,Key Lab Tree, Dongxiaofu 1, Beijing 100091, Peoples R China;[2]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, 26 Hexing Rd, Harbin 150040, Heilongjiang, Peoples R China

年份：2018

卷号：19

期号：1

外文期刊名：BMC GENETICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000445124900001)】；

基金：This work was supported by Forestry Industry Research Special Funds for Public Welfare Projects [201504101]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：Long non-coding RNAs; Woody plant; Floral transition; RNA-sequencing; Catalpa bungei

摘要：Background: Long non-coding RNAs (lncRNAs) have crucial roles in various biological regulatory processes. However, the study of lncRNAs is limited in woody plants. Catalpa bungei is a valuable ornamental tree with a long cultivation history in China, and a deeper understanding of the floral transition mechanism in C. bungei would be interesting from both economic and scientific perspectives. Results: In this study, we categorized C. bungei buds from early flowering (EF) and normal flowering (NF) varieties into three consecutive developmental stages. These buds were used to systematically study lncRNAs during floral transition using high-throughput sequencing to identify molecular regulatory networks. Quantitative real-time PCR was performed to study RNA expression changes in different stages. In total, 12,532 lncRNAs and 26,936 messenger RNAs (mRNAs) were detected. Moreover, 680 differentially expressed genes and 817 differentially expressed lncRNAs were detected during the initiation of floral transition. The results highlight the mRNAs and lncRNAs that may be involved in floral transition, as well as the many lncRNAs serving as microRNA precursors. We predicted the functions of lncRNAs by analysing the relationships between lncRNAs and mRNAs. Seven lncRNA-mRNA interaction pairs may participate in floral transition. Conclusions: This study is the first to identify lncRNAs and their potential functions in floral transition, providing a starting point for detailed determination of the functions of lncRNAs in C. bungei.