文献类型：期刊文献

英文题名：Antifungal Activities of Volatile Secondary Metabolites of Four Diaporthe Strains Isolated from Catharanthus roseus

作者：Yan, Dong-Hui[1] Song, Xiaoyu[1] Li, Hongchang[1] Luo, Tushou[2] Dou, Guiming[1] Strobel, Gary[3]

通信作者：Yan, DH[1];Strobel, G[2]

机构：[1]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[3]Montana State Univ, Dept Plant Sci, Bozeman, MT 59717 USA

年份：2018

卷号：4

期号：2

外文期刊名：JOURNAL OF FUNGI

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000451385000022)】；

基金：This work was supported by Fundamental Research Funds of CAF (CAFYBB2017MA010, to D.-H.Y.). We thank Han Xu (Research Institute of Tropical Forestry, Chinese Academy of Forestry) for kindly helping with host plant identification, and thank Kaiying Wang (Research Institute of Forest Ecology, Environment and Protection) for taking part in the work on sample collection and fungal isolation. The authors sincerely appreciate the three peer reviewers' valued comments on the paper.

语种：英文

外文关键词：endophytic fungi; Diaporthe spp.; Catharanthus roseus; volatile organic compounds (VOCs); antifungal bioactivity; inhibition; terpene; pathogens

摘要：Four endophytic fungi were isolated from the medicinal plant, Catharanthus roseus, and were identified as Diaporthe spp. with partial translation elongation factor 1-alpha (TEF1), beta-tubulin (TUB), histone H3 (HIS), calmodulin (CAL) genes, and rDNA internal transcribed spacer (ITS) region (TEF1-TUB-HIS-CAL-ITS) multigene phylogeny suggested for species delimitation in the Diaporthe genus. Each fungus produces a unique mixture of volatile organic compounds (VOCs) with an abundant mixture of terpenoids analyzed by headspace solid-phase microextraction (SPME) fiber-GC/MS. These tentatively-detected terpenes included alpha-muurolene, beta-phellandrene, gamma-terpinene, and alpha-thujene, as well as other minor terpenoids, including caryophyllene, patchoulene, cedrene, 2-carene, and thujone. The volatile metabolites of each isolate showed antifungal properties against a wide range of plant pathogenic test fungi and oomycetes, including Alternaria alternata, Botrytis cinerea, Colletotrichum gloeosporioides, Fusarium graminearum, and Phytophthora cinnamomi. The growth inhibition of the pathogens varied between 10% and 60% within 72 h of exposure. To our knowledge, the endophytic Diaporthe-like strains are first reported from Catharanthus roseus. VOCs produced by each strain of the endophytic Diaporthe fungi were unique components with dominant monoterpenes comparing to known Diaporthe fungal VOCs. A discussion is presented on the inhibitive bioactivities of secondary metabolites among endophytic Diaporthe fungi and this medicinal plant.