文献类型：期刊文献

英文题名：De novo transcriptome assembly, development of EST-SSR markers and population genetic analyses for the desert biomass willow, Salix psammophila

作者：Jia, Huixia[1,2] Yang, Haifeng[3] Sun, Pei[1] Li, Jianbo[1] Zhang, Jin[1] Guo, Yinghua[1] Han, Xiaojiao[1] Zhang, Guosheng[3] Lu, Mengzhu[1,2] Hu, Jianjun[1,2]

第一作者：Jia, Huixia

通信作者：Hu, JJ[1];Hu, JJ[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing 210037, Jiangsu, Peoples R China;[3]Inner Mongolia Agr Univ, Coll Forestry, Hohhot 010019, Peoples R China

年份：2016

卷号：6

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000389982800001)】；

基金：We are very grateful to two anonymous reviewers for their extremely constructive suggestions that improved this manuscript. This work was supported by National Nonprofit Institute Research Grant of CAF (CAFYBB2014ZX001-4) and the National High-Tech R&D Program (2011AA100201) to J.J.H., and the National Natural Science Foundation of China (31160167) to G.S.Z. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：Salix psammophila, a sandy shrub known as desert willow, is regarded as a potential biomass feedstock and plays an important role in maintaining local ecosystems. However, a lack of genomic data and efficient molecular markers limit the study of its population evolution and genetic breeding. In this study, chromosome counts, flow cytometry and SSR analyses indicated that S. psammophila is tetraploid. A total of 6,346 EST-SSRs were detected based on 71,458 de novo assembled unigenes from transcriptome data. Twenty-seven EST-SSR markers were developed to evaluate the genetic diversity and population structure of S. psammophila from eight natural populations in Northern China. High levels of genetic diversity (mean 10.63 alleles per locus; mean HE 0.689) were dectected in S. psammophila. The weak population structure and little genetic differentiation (pairwise F-ST = 0.006-0.016) were found among Population 1-Population 7 (Pop1-Pop7; Inner Mongolia and Shaanxi), but Pop8 (Ningxia) was clearly separated from Pop1-Pop7 and moderate differentiation (pairwise F-ST = 0.045-0.055) was detected between them, which may be influenced by local habitat conditions. Molecular variance analyses indicated that most of the genetic variation (94.27%) existed within populations. These results provide valuable genetic informations for natural resource conservation and breeding programme optimisation of S. psammophila.