文献类型：期刊文献

中文题名：太空环境诱导的草地早熟禾皱叶突变体蛋白质组学研究

英文题名：PROTEOMIC ANALYSIS OF A WINNKLE-LEAF MUTANT IN Poa pratensis L.INDUCED BY AEROSPACE ENVIRONMENT

作者：韩蕾[1] 孙振元[1] 巨关升[1] 钱永强[1] 彭镇华[1]

第一作者：韩蕾

机构：[1]中国林业科学研究院林业研究所/国家林业局林木培育重点实验室

年份：2005

卷号：19

期号：6

起止页码：417-420

中文期刊名：核农学报

外文期刊名：Acta Agriculturae Nucleatae Sinica

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家"863"计划:优质抗逆草坪草早熟禾等新品种选育(2002AA241061)

语种：中文

中文关键词：太空环境;草地早熟禾;皱叶突变株系;蛋白质组学

外文关键词：aerospace condition; Poa pratensis L. ; winnkle-leaf mutant; protemics

分类号：S688.4;Q51

摘要：“神舟3号”飞船搭载草地早熟禾(Poa pratensisL.)‘Nassau’干种子,地面种植后筛选出皱叶突变株系PM2。利用蛋白质组学技术对PM2突变株系叶片中表达的蛋白质进行解析(未搭载植株为对照),发现基本保持不变的蛋白质点有181个,上调节表达蛋白质点有15个,下调节表达蛋白质点有15个,失去表达蛋白质点有116个。选择19个蛋白质点进行肽质谱分析,同源性查询后其中10个蛋白质点得到阳性结果,且PM2-058为谷氨酸合成酶类似蛋白,PM2-175为磷酸丙糖异构酶类似蛋白;CK-103为ATP酶依赖型26S蛋白酶体类似蛋白;CK-330为1,5二磷酸核酮糖羧化酶/加氧酶类似蛋白;CK-376为硫氧还蛋白M前体类似蛋白;CK-391为二磷酸核酮糖羧化酶类似蛋白。
The dry seeds of Poa pratensis L. variety ＇ Nassau＇ were carried by spaceship SZ-3 as treatments and the seeds which on the earth as CK. A winnkle-leaf mutant which named PM2 was selected for the analysis. Proteins expressed in leaf of CK and PM2 were compared and profiled with the techniques of proteomics. There are 181 proteins maintained no changes, 15 proteins were up-regulated and 15 proteins were down-regulated, and 116 proteins were lost. 19 proteins were chosen to make pdypeptide fingerprint and homelogy analysis. It indicated that ,10 of them were positive results and protein PM2-058 was close to glutamine synthetase, PM2-175 was close to triosephosphate isomerase, CK-103 was close to 26S proteasome regulatory complex ATPase, CK-330 was close to ribulose 1,5 biphosphate carboxylase/oxygenase, CK-376 was close to thioredoxin M precursor, CK-391 was close to ribulose bisphosphate carboxylase.