文献类型：期刊文献

中文题名：‘辽宁2号’核桃品种矮化机制初探

英文题名：Dwarfing Mechanism of Dwarfed Walnut Cultivar ‘Liaoning 2'

作者：宋晓波[1] 陈凌娜[1] 王红霞[2] 裴东[1]

第一作者：宋晓波

机构：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091;[2]河北农业大学山区研究所,河北保定071001

年份：2015

卷号：28

期号：6

起止页码：910-914

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基金项目“核桃埋干复幼促根过程中生长素响应基因的CpG岛甲基化重编程研究(31171933)”

语种：中文

中文关键词：核桃;辽宁2号;矮化;SSR位点;JrGAI

外文关键词：walnut ; ＇ Liaoning 2＇ ; dwarfing ; SSR loci ; JrGAI

分类号：S792.13

摘要：核桃是我国重要的经济树种,在发展山区经济、退耕还林和保障粮油安全等方面扮演重要的角色。在植物学和树木分类学中核桃属植物通常被定义为高达乔木,自然生长情况下,成年树树高可达25~30 m[1-2]。
＇ Liaoning 2＇ is a dwarf walnut variety bred by Chinese scientists through artificial hybridization, but lit- tle is known about its dwarfing mechanism. In this study, the dwarfing mechanism of ＇ Liaoning 2＇ is discussed through the comparison of biological characteristics, genomics and the expression of growth-related gene JrGAI,with a growth normally, closely related （ half-sib families） variety of ＇ Liaoning 1 ＇ as control. By observing the phenolo- gy and tree phenotypes, it was found that there was no significant difference in phenological phase between ＇ Liaon- ing 1＇ and ＇ Liaoning 2＇ , but ＇ Liaoning 2＇ was obviously dwarf, the height, crown and trunk diameter were smal- ler, the average length of internode reduced significantly, but there was no significant difference in the number of development branch internode. The dwarf phenotype of ＇ Liaoning 2＇ is mainly caused by inter-shortening. The qRT-PCR technique was performed to analyze the expression of GA negative regulation gene JrGAI and the results showed that during the shoot development, the expression of JrGAI gene exhibited decreasing expression in the shoot rapid growing period in both varieties and the decline in ＇ Liaoning I ＇ was remarkably reduced. The SSR analysis with 25 pairs of primers showed that SSR loci of two varieties were different, especially the homozygosity of ＇ Liaon- ing 2＇ SSR loci was higher. It is concluded that different expressions of JrGAI gene and homozygosity of SSR loci could be important internal reasons of dwarf phenotype of ＇ Eiaoning 2＇.