文献类型：期刊文献

中文题名：紫斑牡丹‘书生捧墨’的体胚诱导与发生

英文题名：Inducement and development of somatic embryos in Paeonia rockii ‘Shu Sheng Peng Mo’.

作者：周秀梅[1,2] 成仿云[1] 钟原[1] 齐力旺[3]

第一作者：周秀梅

机构：[1]北京林业大学园林学院 国家花卉工程技术研究中心,100083;[2]河南科技学院园林学院,453003;[3]中国林业科学研究院林业研究所

年份：2009

期号：2

起止页码：151-154

中文期刊名：北京林业大学学报

外文期刊名：Journal of Beijing Forestry University

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：“十一五”国家科技支撑计划项目(2006BAD01A1801);国家自然科学基金项目(30170784)

语种：中文

中文关键词：紫斑牡丹;体胚发生;正交设计

外文关键词：Paeonia rockii;somatic embryogenesis;orthogonal experimental design

分类号：S685.11

摘要：为筛选适于紫斑牡丹体胚发生的诱导培养基,以‘书生捧墨’花后90 d的种胚为外植体,采用L9(34)正交设计,研究了诱导培养基中基本培养基、蔗糖、BAP和NAA 4个因素及其组合对体胚发生的影响。结果表明:4个因素对体胚诱导率的影响均达到极显著水平,且以NAA对其影响最大,基本培养基和BAP次之,蔗糖的影响最小;4个因素的最佳组合为:改良MS+BAP 0.5 mg/L+NAA 0 mg/L+蔗糖100.0 g/L;经包含该组合的培养基培养70 d,体胚诱导率达63.3%,并获得了体胚再生植株。
In order to screen optimal medium for inducing the somatic embryogenesis in Paeonia rockii hybrids,the effects of basal media,sucrose,BAP and NAA as well as their combinations on the inducement and development of somatic embryos were investigated from the embryos obtained at the 90（th） day after flowering based on the orthogonal design L_9（3~4）.The results showed that all four factors had highly significant effects on the percentage of the explants with somatic embryos.The effect of NAA was the most significant,followed by those of basal media and BAP,and the effect of sucrose was the least significant.The optimum combination of the four factors was revised MS＋BAP 0.5 mg/L＋NAA 0 mg/L＋Sucrose 100.0 g/L.Somatic embryos and regenerated plantlets were obtained in 63.3%of the explants cultured on the media with the optimum combination for 70 days.