文献类型：期刊文献

英文题名：Characterization of pollen tube development in distant hybridization of Chinese cork oak (Quercus variabilis L.)

作者：Ke, Meng[1] Si, Huayu[1] Qi, Yongliang[1] Sun, Yuhan[1] El-Kassaby, Yousry A.[2] Wu, Zhiyong[3] Li, Shian[3] Liu, Kelin[3] Yu, Hai[4] Hu, Ruiyang[4] Li, Yun[1]

第一作者：Ke, Meng

通信作者：Li, Y[1]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Technol, Engn Technol Res Ctr Black Locust Natl Forestry &, Natl Engn Res Ctr Tree Breeding & Ecol Restorat,St, Beijing 100083, Peoples R China;[2]Univ British Columbia, Fac Forestry, Dept Forest & Conservat Sci, 2424 Main Mall, Vancouver, BC V6T 1Z4, Canada;[3]Beijing Green Space Maintenance & Management Serv, Beijing 102200, Peoples R China;[4]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102399, Peoples R China

年份：2023

卷号：258

期号：6

外文期刊名：PLANTA

收录：;Scopus(收录号：2-s2.0-85175717741);WOS:【SCI-EXPANDED(收录号:WOS:001095294600001)】；

基金：This work was funded by the National Key R&D Program of China (2021YFD2200302) and the Fundamental Research Funds for the Central Universities (PTYX202338). We thank the other students in our lab for their assistance.

语种：英文

外文关键词：Fagaceae; Heterospecific pollen; Outcross- and interspecific-pollination; Pollen-pistil interactions; Q. mongolica

摘要：Quercus is the foundation species in the Northern Hemisphere, with extreme prevalence for interspecific hybridization. It is not yet entirely understood whether or how the pollen tube-female tissue interaction contributes to the "extensive hybridization" in oaks. Pollen storage conditions correlate with distant hybridization. We conducted hybridization experiments with Q. variabilis as female and Q. variabilis and Q. mongolica as male parents. And the differences in pollen tube (PT) development between intra- and distant interspecific hybridization were studied by fluorescence microscopy and scanning electron microscopy (SEM). Our results showed that -20 C-degrees allowed pollen of both species to maintain some viability. Both Q. variabilis and Q. mongolica pollen germinated profusely on the stigmas. SEM results indicated that in the intraspecific hybridization, Q. variabilis pollen started to germinate at 6 h after pollination (hap), PTs elongated significantly at 12 hap, and entered the stigma at 24 hap. By contrast, Q. mongolica pollen germinated at 15 hap, and the PTs entered the stigma at 27 hap. By fluorescence microscopical studies it was observed that some PTs of Q. variabilis gathered at the style-joining at 96 hap, unlike the Q. mongolica which reached the style junction at 144 hap. The above results indicate that the abundant germination of heterologous pollen (HP) on the stigma and the "Feeble specificity recognition" of the stigma and transmitting tract to HP may create opportunities for the "extensive hybridization" of oaks. This work provides a sexual developmental reference for clarifying the causes of Quercus "extensive hybridization".