文献类型：期刊文献

英文题名：Isolation and characterization of larch BABY BOOM2 and its regulation of adventitious root development

作者：Wang, Hongming[1,2] Li, Kuipeng[3] Sun, Xiaomei[1,2] Xie, Yunhui[1,2] Han, Xuemin[1] Zhang, Shougong[1,2]

通信作者：Zhang, SG[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Xiangshan Rd, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Xiangshan Rd, Beijing 100091, Peoples R China;[3]Guangxi Forestry Res Inst, 23 Yongwu Rd, Nanning, Guangxi Provinc, Peoples R China

年份：2019

卷号：690

起止页码：90-98

外文期刊名：GENE

收录：;Scopus(收录号：2-s2.0-85059384608);WOS:【SCI-EXPANDED(收录号:WOS:000458707600011)】；

基金：This work was supported by "the Forestry Industry Research Special Funds for Public Welfare Projects (201504104)". We thank Sarah Williams, PhD, from Liwen Bianji, Edanz Group China (www.liwenbianji.cn), for editing the English text of a draft of this manuscript.

语种：英文

外文关键词：Conifers; Adventitious root; Gene expression; Subcellular localization; Genetic transformation

摘要：The BABY BOOM2 gene, designated LIcBBM2, and its promoter were isolated from hybrid larch (Larix kaempferi x L. olgensis). The open reading frame of LkBBM2 was 2574 bp, encoding 857 amino acids. The LkBBM2 protein contains two AP2 DNA binding domains and a BBM specific motif, but lacks the euANT5 motif common to AP2 family members. The LkBBM2 promoter contains several hormone response and root-specific expression elements. LkBBM2 expression was significantly higher in larch adventitious roots (ARs) than in stems, leaves or stem tips, and increased after auxin treatment. The fused protein LkBBM2-GFP was localized in both the nucleus and cytoplasm whereas LkBBM1-GFP was only localized in the nucleus. Over-expression of LkBBM2 and LkBBM1 in Arabidopsis significantly elongated the roots. Furthermore, over-expression those two genes in the hybrid poplar (Populus alba x P. glandulosa) significantly increased ARs number. We speculated that these two genes regulate AR development.