文献类型：期刊文献

英文题名：Association Genetics Identifies Single Nucleotide Polymorphisms Related to Kernel Oil Content and Quality in Camellia oleifera

作者：Lin, Ping[1,2] Yin, Hengfu[1,2] Yan, Chao[1,2,3] Yao, Xiaohua[1,2] Wang, Kailiang[1,2]

第一作者：林萍

通信作者：Lin, P[1];Wang, KL[1];Lin, P[2];Wang, KL[2]|[a000566e35c9f8bb73f29]林萍;

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Forest Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Fenyi 336600, Peoples R China

年份：2019

卷号：67

期号：9

起止页码：2547-2562

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000460996800014)】；

基金：This work was supported by National Natural Science Foundation of China (No.31770719, No. 31400580) and the Fundamental Research Funds for the Central Nonprofit Research Institution of Chinese Academy of Forestry (CAFYBB2016SZ001, CAFYBB2014QB037).

语种：英文

外文关键词：candidate-gene-based association mapping; Camellia oleifera; single nucleotide polymorphisms (SNPs); oil content (OC) and fatty acid composition; stearoyl-ACP desaturase gene (CoSAD) and Delta 12 (omega 6)-desaturase gene (Cofad2)

摘要：Camellia oleifera, as an important nonwood tree species for seed oil in China, has received enormous attention owing to its high unsaturated fatty acid contents benefited to human health. It is necessary to examine allelic diversity of key genes that are associated with oil production in C. oleifera cultivars with a large variation of fatty acid compositions. In this study, we performed the association analysis between four key genes (two CoSAD and two Cofad2) coding fatty acid desaturases and traits including oil content and fatty acid composition. We identified two single nucleotide insertion-deletion (InDel) and 362 single-nucleotide polymorphisms (SNPs) within the four candidate genes by sequencing an association population (216 accessions). Single-marker (or haplotype) and traits association tests were conducted by linkage disequilibrium (LD) approaches to detect significant marker-trait associations. Validation population (279 hybrid individuals from six full-sibs families) studies were performed to validate the function of allelic variations significantly associated. In all, 90 single marker-trait and one haplotype-trait associations were significant in association population, and these loci explained 1.87-17.93% proportion of the corresponding phenotypic variance. Further, six SNP marker-trait associations (Q < 0.10) from Cofad2-A, CoSAD1, and CoSAD2 were successfully validated in the validation population. The SNP markers identified in this study can potentially be applied for future marker-assisted selection to improve oil content and quality in C. oleifera.