文献类型：期刊文献

英文题名：Symbiotic fungi in roots of Artemisia annua with special reference to endophytic colonizers

作者：Yuan, Z. L.[1,2] Chen, Y. C.[1] Ma, X. J.[3]

第一作者：袁志林;Yuan, Z. L.

通信作者：Yuan, ZL[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Peoples R China;[2]Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310029, Zhejiang, Peoples R China;[3]Chinese Acad Med Sci, Guangxi Branch, Inst Med Plant, Inst Guangxi Med Plant,Guangxi Bot Garden Med Pla, Nanning 530023, Peoples R China

年份：2011

卷号：145

期号：2

起止页码：495-502

外文期刊名：PLANT BIOSYSTEMS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000295684800027)】；

语种：英文

外文关键词：Artemisia annua; dark septate endophytes; arbuscular mycorrhizal fungi

摘要：Advances on plant-fungal interactions reveal that root symbiotic fungi actively modulate host growth, resistance response and secondary metabolism. Artemisia annua has been widely recognized as an important medicinal plant for artemisinin production, yet little is known about the fungal consortium associated with roots of A. annua. In this article, microscopic and culture-dependant methods were used to evaluate the identity and taxonomic affinities of root symbiotic fungi. Morphological evidence confirmed that arbuscular mycorrhizal fungi were dominant fungal group in naturally regenerated roots, but low colonization frequency in planted roots. Dark septate endophytes (DSEs) were easily found, which were characterized with dark pigmented hypha and a sclerotium-like structure in root cortex, and other endophytic fungi also occurred. A total of 36 isolates were recovered. Combined morphological and molecular identification (based on ITS sequences) determined 21 fungal taxa (genotype), which were placed into numerous lineages of Ascomycota. The best BLAST match indicated that almost half of total taxa were closely related to undescribed fungi, some of them may act as novel DSEs but experimental data were warranted. Interestingly, remarkable difference of fungal community associated with two types of roots was examined and no culturable fungi overlapped. Our findings provide some additional evidence that DSEs and other root endophytes may be as common as mycorrhizal fungi. Recovered fungi as raw materials for bioassay of endophytes-mediated promotion of artemisinin content in A. annua will be conducted in further research.