文献类型：期刊文献

英文题名：Clustered NBS-LRR and defensive metabolism genes define a major insect resistance locus in Salix suchowensis

作者：Fu, Wei[1] Wang, Siyu[2] Zheng, Baoyu[2] Hu, Jianjun[3] Chen, Yingnan[1] Jiang, Anna[2] Wei, Suyun[1,2]

第一作者：Fu, Wei

通信作者：Wei, SY[1]

机构：[1]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Key Lab Tree Genet & Biotechnol Educ Dept China, State Key Lab Tree Genet & Breeding, Nanjing 210037, Peoples R China;[2]Nanjing Forestry Univ, Coll Informat Sci & Technol & Artificial Intellige, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2026

卷号：239

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20255019687909);Scopus(收录号：2-s2.0-105024218083);WOS:【SCI-EXPANDED(收录号:WOS:001639806100004)】；

基金：The work is supported by the National Key Research and Develop-ment Plan of China (2021YFD2201100) and the National Natural Sci-ence Foundation of China (32471900) .

语种：英文

外文关键词：Insect resistance; Salix suchowensis; NBS-LRR gene cluster; Defensive secondary metabolism; Plant defensin

摘要：Salix suchowensis is an important bioenergy and ecological restoration species that frequently suffers severe damage from folivorous insects. To uncover its genetic basis of resistance, this study integrated whole-genome resequencing of resistant and susceptible individuals with bulk segregant analysis and mapped a major resistance locus in a 3.4 megabase region on chromosome 19. This region contains 186 protein-coding genes enriched in pathways related to insect recognition, signal transduction, and secondary metabolism. Notably, it harbors 63 NBS-LRR genes and a cluster of 31 genes associated with defense metabolite regulation, forming a functionally dense resistance module. Transcriptome profiling further identified four highly expressed genes within this region that are strongly associated with insect resistance. Specially, SuTNL is linked to immune recognition, SuSAUR to auxin signaling, SuHCT to phenylpropanoid metabolism, and SuPDF to direct insecticidal defense. In particular, the expression level of SuPDF was 64 times higher in resistant individuals, suggesting that it acts as a major effector conferring insect resistance. Overall, this study precisely defines a functionally dense resistance locus on chromosome 19, reveals a coordinated network between NBS-LRR gene clusters and defense metabolism, and identifies SuPDF as a pivotal gene conferring resistance. These findings provide valuable molecular insights and breeding targets for pest resistance improvement in woody plants.