文献类型：期刊文献

英文题名：S-nitrosylation of thaumatin-like protein in Ziziphus jujuba and its roles in chilling stress and pathogen stress responses

作者：Gao, Yizhou[1] Guo, Ting[1] Liu, Lingling[1] Li, Li[2] Wu, Zhengbao[3] Jiang, Yunhong[4] Ban, Zhaojun[1] Yang, Zhen[5]

第一作者：Gao, Yizhou

通信作者：Ban, ZJ[1];Yang, Z[2]

机构：[1]Zhejiang Univ Sci & Technol, Zhejiang Prov Collaborat Innovat Ctr Agr Biol Reso, Sch Biol & Chem Engn, Hangzhou 310023, Peoples R China;[2]Zhejiang Univ, Coll Biosyst Engn & Food Sci, Key Lab Agroprod Postharvest Handling, Minist Agr & Rural Affairs, Hangzhou 310058, Peoples R China;[3]Xinjiang Acad Forestry Sci, Econ Forest Res Inst, Urumqi 830000, Peoples R China;[4]Northumbria Univ, Dept Appl Sci, Newcastle Upon Tyne NE1 8ST, England;[5]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China

年份：2025

卷号：230

外文期刊名：POSTHARVEST BIOLOGY AND TECHNOLOGY

收录：;Scopus(收录号：2-s2.0-105010617491);WOS:【SCI-EXPANDED(收录号:WOS:001540309200001)】；

基金：This research received funding from the National Natural Science Foundation of China [grant number 32172268], Special Fund Project of Xinjiang Jujube Industry Technology System [grant number XJCYTX-01-02], the Fundamental Research Funds of CAF (grant number CAFYBB2023MB003), International Exchanges Funds offered by the Royal Society [grant number IEC\NSFC\233076].

语种：英文

外文关键词：Jujube; Chilling injury; Nitric oxide; S-nitrosylation; Thaumatin-like protein

摘要：Nitric oxide is a key regulator in alleviating chilling injury in postharvest fruits through S-nitrosylation of functional proteins. This study identified ZjTLP, a thaumatin-like protein in jujube, as a critical target of Snitrosylation at cysteine residues Cys31, Cys195, and Cys212. Through bioinformatics and molecular simulations, we revealed structural stabilization and increased solvent accessibility of ZjTLP protein under S-nitrosylation. Functional assays demonstrated ZjTLP's beta-1,3-glucanase activity and its extracellular secretion properties. ZjTLP gene expression was modulated by NO treatment, low-temperature stress, and fungal infection, highlighting its potential role in cold stress mitigation and pathogen defense.