文献类型：期刊文献

英文题名：Genome-Wide Identification of WOX Gene Family and Expression Analysis during Rejuvenational Rhizogenesis in Walnut (Juglans regia L.)

作者：Chang, Yingying[1] Song, Xiaobo[1] Zhang, Qixiang[2] Liu, Hao[3] Bai, Yongchao[1] Lei, Xiashuo[1] Pei, Dong[1]

第一作者：Chang, Yingying

通信作者：Pei, D[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry &, Beijing 100091, Peoples R China;[2]Zhejiang A&F Univ, Sch Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Nurturing Stn, Linan 311300, Peoples R China;[3]Tianjin Inst Forestry & Pomol, Tianjin 300384, Peoples R China

年份：2020

卷号：11

期号：1

外文期刊名：FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000513184500016)】；

基金：This research was funded by the National Key Research and Development Program of China (Grant: 2018YFD1000604), the National Natural Science Foundation of China (No. 31670682), and the National Natural Science Foundation of China (No. 31672126).

语种：英文

外文关键词：WOX family; Juglans regia; rejuvenation; ARF

摘要：Rejuvenation is an efficient approach used in the cuttings of trees and horticultural crops, to improve their rooting ability, especially in difficult-to-root trees. WOX gene family members are involved in cell-fate transformation through balancing the maintenance and proliferation of the stem cells. However, there are no reports about the WOX gene family in Walnut (Juglans regia L.) and its relationship between rejuvenation and adventitious roots formation (ARF). Here, a genome-wide identification of JrWOX genes and their physical and chemical properties, phylogeny, and expression profiles in different organs and during rejuvenation-induced ARF is reported. The phenotype and histology characteristics of mature and rejuvenated cuttings (Mc and Rc) are also observed. In this study, 12 genes were identified and clustered into three groups based on phylogenetics, special domains, and conserved motifs. The gene structures and conserved motifs were relatively conserved, while the 12 sequences of the JrWOXs domain were diversified. Gene expression in root, stem, leaf, female flower, immature fruit, and zygotic embryo revealed that the expression levels of JrWOX4a, JrWOX4b, JrWOX5, JrWOX11, and JrWOX13 in the root were significantly higher than those of other JrWOXs, while only the expression of JrWOX11 was exclusive to the root organ. Additionally, rejuvenation treatment significantly induced almost all JrWOX genes, except JrWOX4a, JrWOX4b, and JrWOX13 (Rc 0 vs. Mc 0). During the ARF process, the transcripts of JrWOX11 and JrWOX5 were consecutively increased on a significance level; in contrast, the transcription levels of the other JrWOXs decreased or changed insignificantly. The phenotype and histology observation indicate that rejuvenation treatment made the base of the stem expand and reduced the thickness and density of sclerenchyma between the cortex and phloem. This might provide the conditions for the formation of new meristem niches. The results provided insight into the JrWOX genes' general characteristics and their roles in rejuvenation-induced ARF.