文献类型：期刊文献

英文题名：Integrated Analysis of Phenotypic and Transcriptomic Variation in an F1 Population of Oil-Camellia Reveals the Major Genes Controlling Seed Size and Oil Accumulation

作者：Liu, Linxiu[1,2,3] Sheng, Yu[1,3] Zhang, Yunbin[4] Xie, Xinru[1,3] Chen, Juanjuan[1,3] Wang, Jingfei[4] Pan, Huanhuan[1,3] Huang, Hu[1,3] Cao, Xun[1,3] Xu, Jing[1,3] Zhuo, Renying[1,3] Yao, Xiaohua[1,3]

第一作者：Liu, Linxiu

机构：[1] Key Laboratory of Tree Breeding of Zhejiang Province, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Zhejiang, Hangzhou, 311400, China; [2] Faculty of Forestry, Nanjing Forestry University, Jiangsu, Nanjing, 210000, China; [3] State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, China; [4] Mount Huangshan Forestry Research Institute, Huangshan, 245099, China

年份：2024

外文期刊名：SSRN

收录：EI(收录号：20240304049)

语种：英文

外文关键词：Oils and fats - Transcription

摘要：Oil-Camellia, a prominent oil-producing tree, is extensively cultivated for production of edible oil, renowned for its high-quality and health benefits. Seed size and oil content serve as primary objectives in hybrid breeding of seed oil yield, which are intricately controlled by complex networks. However, the mechanism underlying the coordinated regulation of seed size and oil content in oil-Camellia, as well as the candidate genes involved, are not fully understood yet. Here, we developed a cross population derived from CL4 and XG, which exhibited notable variations in yield-related traits. Analysis of traits across populations with different ploidy levels indicated that oil content and seed size in certain population increasing by over 2 folds. Additionally, comparative transcriptome analysis was conducted on seed kernels from CL4 and XG across four distinct developmental stages to identify candidate genes that regulate seed development and influence seed oil content. The dynamic expression patterns, along with corresponding phenotypic traits, revealed that Oleosin5 and ACCase α-subunit could be key regulatory genes, simultaneously regulating seed size and oil content accumulation in oil-Camellia, with highly expression levels in S2 or a gradual increase from S1 to S3. Furthermore, the ABA transduction pathway, expansion protein, and transcription factors showed co-expression with the key genes, defining a regulatory pathway centered around Oleosin5 and ACCase α-subunit that controls the molecular mechanisms underlying the accumulation of seed oil content. Our findings not only provide substantial evidence for the significant role of hybridization in the screening of modified population, but also identify a series of genes that govern the two primary determinants of yield in oil-Camellia. This research facilitates our understanding of the molecular basis of oil-Camellia yield and lays a foundation for integrating traditional breeding with molecular breeding to improve the yield of oil-Camellia. ? 2024, The Authors. All rights reserved.