文献类型：期刊文献

英文题名：Overexpressing the Sedum alfredii Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis

作者：Li, Zhen[1,2] Han, Xiaojiao[1,2] Song, Xixi[1,2] Zhang, Yunxing[1,2] Jiang, Jing[1,2] Han, Qiang[1,2] Liu, Mingying[1,2] Qiao, Guirong[1,2] Zhuo, Renying[1,2]

通信作者：Zhuo, RY[1];Zhuo, RY[2]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou, Zhejiang, Peoples R China

年份：2017

卷号：8

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000403307600001)】；

基金：This work was supported by the National High Technology Research and Development Program of China (No. 2013AA102701-3), the National Non-profit Institute Research Grant of CAF (No. TGB2013008 and RISF2014010), and the National Natural Science Foundation of China (No. 31200465).

语种：英文

外文关键词：Cd stress; co-expression network; Cu/Zn SOD; oxidative stress; Sedum alfredii

摘要：Superoxide dismutase (SOD) is a very important reactive oxygen species (ROS)scavenging enzyme. In this study, the functions of a Cu/Zn SOD gene (SaCu/Zn SOD), from Sedum alfredii, a cadmium (Cd)/zinc/lead co-hyperaccumulator of the Crassulaceae, was characterized. The expression of SaCu/Zn SOD was induced by Cd stress. Compared with wild-type (WT) plants, overexpression of SaCu/Zn SOD gene in transgenic Arabidopsis plants enhanced the antioxidative defense capacity, including SOD and peroxidase activities. Additionally, it reduced the damage associated with the overproduction of hydrogen peroxide (H2O2) and superoxide radicals (O-2(center dot-)). The influence of Cd stress on ion flux across the root surface showed that overexpressing SaCu/Zn SOD in transgenic Arabidopsis plants has greater Cd uptake capacity existed in roots. A co-expression network based on microarray data showed possible oxidative regulation in Arabidopsis after Cd-induced oxidative stress, suggesting that SaCu/Zn SOD may participate in this network and enhance ROS-scavenging capability under Cd stress. Taken together, these results suggest that overexpressing SaCu/Zn SOD increased oxidative stress resistance in transgenic Arabidopsis and provide useful information for understanding the role of SaCu/Zn SOD in response to abiotic stress.