文献类型：期刊文献

中文题名：西南桦花粉低温贮藏试验初报

英文题名：Preliminary Report on Storage under Low Temperature of Betula alnoides Pollen

作者：程伟[1] 赵志刚[1] 郭俊杰[1] 刘宝[1] 曾杰[1] 赖家业[2]

第一作者：程伟

机构：[1]中国林业科学研究院热带林业研究所;[2]广西大学林学院

年份：2007

卷号：27

期号：6

起止页码：49-52

中文期刊名：浙江林业科技

外文期刊名：Journal of Zhejiang Forestry Science and Technology

收录：北大核心:【北大核心2004】；

基金：国家"十一五"科技支撑项目"优质珍贵用材树种桦木;水曲柳新品种选育"专题(2006BAD01A1603)中"优质珍贵用材树种西南桦新品种选育"子专题的部分内容

语种：中文

中文关键词：西南桦;低温贮藏;花粉活力;花粉含水量

外文关键词：Betula alnoides; low temperature storage; pollen vigour; pollen moisture content

分类号：S722.3

摘要：从花粉干燥、真空和低温等方面对西南桦花粉贮藏进行了初步研究，结果表明：西南桦花粉属干燥敏感型花粉，干燥易使其失活，其含水量约为20％，用硅胶稍微干燥至含水量约18％后即可用于低温贮藏；西南桦新鲜花粉在室温下仅可存活4d，干燥花粉和新鲜花粉在3℃下贮藏约1个月即失去活力，而在-10℃和-20℃条件下应用干燥花粉真空贮藏，83d后花粉萌发率仍在20％以上，是西南桦花粉贮藏的最佳方案；在-10℃和-20℃下贮藏的花粉在室温（18—30℃）下解冻0．5h即可检测其萌发率。
Study on pollen storage of Betula alnoides by dry, vacuum and low temperature treatment indicated that the pollen was sensitive to dryness which could make inactivation of the pollen. Moisture content of fresh pollen was approximately 20%, and dried to 18% with silica gel for low temperature storage. Fresh pollen could survive only four days under room temperature （18 - 30℃）, both fresh and dried pollen could keep their vigour for about one month under 3℃, while stored under vacuum condition and the temperature of - 10℃ or - 20℃, germination ratio of dried pollen could still have above twenty percent after 83 days. It took only half an hour to unfreeze the pollen stored under - 10℃ or - 20℃ at room temperature for testing their germination rate.