文献类型：期刊文献

英文题名：Multi-omics analysis revealed the mechanism underlying flavonol biosynthesis during petal color formation in Camellia Nitidissima

作者：Feng, Yi[1] Li, Jiyuan[1] Yin, Hengfu[1] Shen, Jian[2] Liu, Weixin[1]

通信作者：Liu, WX[1];Shen, J[2]

机构：[1]Chinese Acad Forestry, Key Lab Tree Breeding Zhejiang Prov, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[2]Jinhua Forestry Technol Promot Stn Zhejiang Prov, Jinhua 321017, Zhejiang, Peoples R China

年份：2024

卷号：24

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-85203375954);WOS:【SCI-EXPANDED(收录号:WOS:001309478700004)】；

基金：Not applicable.

语种：英文

外文关键词：Camellia nitidissima; Flavonol; Multi-omics analysis; CnFLS2; Jasmonate

摘要：Background Camellia nitidissima is a rare, prized camellia species with golden-yellow flowers. It has a high ornamental, medicinal, and economic value. Previous studies have shown substantial flavonol accumulation in C. nitidissima petals during flower formation. However, the mechanisms underlying the golden flower formation in C. nitidissima remain largely unknown. Results We performed an integrative analysis of the transcriptome, proteome, and metabolome of the petals at five flower developmental stages to construct the regulatory network underlying golden flower formation in C. nitidissima. Metabolome analysis revealed the presence of 323 flavonoids, and two flavonols, quercetin glycosides and kaempferol glycosides, were highly accumulated in the golden petals. Transcriptome and proteome sequencing suggested that the flavonol biosynthesis-related genes and proteins upregulated and the anthocyanin and proanthocyanidin biosynthesis-related genes and proteins downregulated in the golden petal stage. Further investigation revealed the involvement of MYBs and bHLHs in flavonoid biosynthesis. Expression analysis showed that flavonol synthase 2 (CnFLS2) was highly expressed in the petals, and its expression positively correlated with flavonol content at all flower developmental stages. Transient overexpression of CnFLS2 in the petals increased flavonol content. Furthermore, correlation analysis showed that the jasmonate (JA) pathways positively correlated with flavonol biosynthesis, and exogenous methyl jasmonate (MeJA) treatment promoted CnFLS2 expression and flavonol accumulation. Conclusions Our findings showed that the JA-CnFLS2 module regulates flavonol biosynthesis during golden petal formation in C. nitidissima.