文献类型：期刊文献

英文题名：Diversity of Soil Acidobacterial Community of Different Land use Types in the Sanjiang Plain, Northeast of China

作者：Sui, Xin[1,2] Zhang, Rongtao[2] Xu, Nan[2] Liu, Yingnan[2] Ni, Hongwei[1] Lei, Jingpin[3] Li, Maihe[4]

第一作者：Sui, Xin

通信作者：Ni, HW[1];Lei, JP[2]

机构：[1]Heilongjiang Univ, Coll Life Sci, Harbin 150080, Heilongjiang, Peoples R China;[2]Heilongjiang Acad Sci, Inst Nat & Ecol, Harbin 150040, Heilongjiang, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[4]Swiss Fed Res Inst WSL, Birmensdorf, Switzerland

年份：2017

卷号：19

期号：5

起止页码：1279-1285

外文期刊名：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY

收录：;Scopus(收录号：2-s2.0-85029711898);WOS:【SCI-EXPANDED(收录号:WOS:000412172700044)】；

基金：We thank the foundation supported of National Natural Science Foundation of China (31470019, 31400429, 31500410); the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2014ZD001). We also appreciate Special Finance Application Technology Project of Heilongjiang Province (STJB16-01; STJB16-03) and Young Innovation Foundation of Heilongjiang Academy of Sciences.

语种：英文

外文关键词：High-throughput sequencing; Soil acidobacteria; beta-diversity; Community structure

摘要：Different perturbation regimes, including disturbance caused by cultivation or the process of natural restoration can have significant effects on the soil acidobacterial community in wetland. The relationship between the community composition and diversity of acidobacteria and perturbation in wetland to quantify the extent of such disturbance related changes in northeast China. We assessed the diversity of acidobacterial communities in nine samples of wetland soil collected from pristine marsh, neighboring cropland and a restoration forest land. High-throughput sequencing of a acidobacteria-specific genomic sequence the internal transcribed spacer (16S rRNA) region was used to identify acidobacterial taxa. We obtained 1, 523, 313 sequences that represented 7881 acidobacterial operational taxonomic units across the three types of sampling sites. Of these, 2847OTUs occurred at all three site types, 814 were shared between farmland and pristine wetland, 206 were shared between forestland and pristine wetland 1459 were shared between farmland and forestland. All sites also hosted unique acidobacterial OTUs with 639 OTUs exclusive to farmland 1616 exclusive to pristine wetland and 300 exclusive to forestland. Sequences were affiliated to 25 acidobacterial subgroups throughout the dataset. Sequence abundance showed that members of Acidobacteria_Gp1, Acidobacteria_Gp3, Acidobacteria_Gp7, Acidobacteria_Gp6, Acidobacteria_Gp2, Acidobacteria_Gp4, Acidobacteria_Gp13 (relative abundance> 1%), which represented an overwhelming proportion of the soil acidobacterial communities and another 18 subgroups with an average relative abundance were< 1%. The dominant subgroups that showed the greatest variation among habitat types. The soil bacterial community diversity decreased from a maximum in farmland, through the wetland, to a minimum in forestland. Canonical correlation analyses demonstrated that land use patterns changing, significantly altered the bacterial community composition of soil. The a-diversity of the soil acidobacterial community was most affected by soil physical and chemical properties, such as pH, soil organic carbon (SOC), total nitrogen (TN), available nitrogen (AN) and available phosphorus (AP). Meanwhile, the soil acidobacterial community composition was significantly affected by pH available phosphorus, nitrogen and organic carbon. Overall, the results from our study showed that the acidobacterial community composition of wetland were affected by SOC, TN, TP and AN. In addition, our results also indicate that cultivation and natural restoration influenced the acidobacterial community structure and diversity. Cultivation can significantly enhance the acidobacterial diversity; however, once the composition of the wetland bacterial community has been altered by cultivated disturbance, it might be difficult to restore to its original state. These findings highlight the importance of effectively managing the soil acidobacterial community to maintain a naturally functioning soil ecosystem. c 2017 Friends Science Publishers