文献类型：期刊文献

英文题名：Isolation of cadmium-resistance and siderophore-producing endophytic bacteria and their potential use for soil cadmium remediation

作者：Li, Yanglong[1,2,3] Wei, Shumeng[1,2,3] Chen, Xiangteng[1,2,3] Dong, Yuhong[1,2,3] Zeng, Mansheng[4] Yan, Chaowu[5] Hou, Lingyu[1,2,3] Jiao, Ruzhen[1,2,3]

通信作者：Jiao, RZ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat, State Forestry Adm, Beijing 100091, Peoples R China;[4]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Fenyi 336600, Peoples R China;[5]Forestry Bur Xinyu City, Xinyu 338000, Jiangxi, Peoples R China

年份：2023

卷号：9

期号：7

外文期刊名：HELIYON

收录：;Scopus(收录号：2-s2.0-85164533845);WOS:【SCI-EXPANDED(收录号:WOS:001059594400001)】；

基金：Acknowledgements This research was supported by the horizontal project "Screening and evaluation of highly enriched plants for remediation of heavy metal contaminated soil" from Standard Technology Engineering (Qingdao) Company Limited.

语种：英文

外文关键词：Cadmium contamination; Cadmium resistance; Endophytic bacterium; Endophyte-assisted phytoremediation; Siderophore; Plant growth promotion

摘要：Endophyte-assisted phytoremediation is an emerging technique for soil heavy metals (HMs) remediation and has become a research focus in the world because of the benefits of endophytes on plant growth and uptake of HMs. In this study, multifunctional endophytic bacteria strains were isolated and screened, and the feasibility of these strains for soil cadmium (Cd) remediation was investigated by soil incubation experiments and pot experiments. All endophytic bacteria were isolated from the roots of woody plants grown on Cd-contaminated soil. Seven endophytic bacteria strains had capacities to tolerate Cd toxicity and produce siderophores, and sequence analysis of the 16S rRNA gene classified these strains as belonging to the genera Burkholderia, Pseudomonas, Pantoea, and Herbaspirillum. All strains were able to produce hydroxamate siderophores (32.40%-91.49%) and had three or more plant growth promoting properties such as phosphorus solubilization, nitrogen fixation, indole acetic acid and 1-aminocyclopropane-1carboxylate deaminase production. They were all strongly resistant to Cd2+ toxicity, with the minimum inhibitory concentration in LB medium ranging from 1.5 mM to 9.0 mM. Except for strain Burkholderia contaminans JLS17, other strains showed decreasing removal rates within continuously elevated Cd2+ concentration of 10-100 mg L-1. Compared with the uninoculated treatment, the inoculation of strains B.contaminans JLS17, Pseudomonas lurida JLS32, and Pantoea endophytica JLS50 effectively increased the concentration of acid-soluble Cd and decreased the concentration of reducible, oxidizable, and residual Cd in the soils of different Cd contamination levels. In pot experiments, inoculation of strains JLS17 and YTG72 significantly (p < 0.05) promoted the growth of above-ground parts and root system of slash pine (Pinus elliottii) under Cd stress. This study provides a valuable biological resource for endophyte-assisted phytoremediation and a theoretical basis for the application of endophytic bacteria for remediation of Cd-contaminated soil.