文献类型：期刊文献

英文题名：Screening of potential chemical marker with interspecific differences in Pterocarpus wood and a spatially-resolved approach to visualize the distribution of the characteristic markers

作者：Liu, Bo[1] Chen, Qian[1] Tang, Lina[1] Zhu, Liming[1] Zou, Xianwu[1] Li, Botao[1] Fan, Wei[2] Fu, Yuejin[1] Lu, Yun[1]

第一作者：刘波

通信作者：Fu, YJ[1];Lu, Y[1];Fan, W[2]

机构：[1]Chinese Acad Forestry, Res Inst Wood Ind, Beijing, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China

年份：2023

卷号：14

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000940337000001)】；

基金：This work was supported financially by the National Natural Science Foundation of China (Grant No. 31870539 and 31870535), the Fundamental Research Funds of the Chinese Academy of Forestry (Grant No. CAFYBB2022ZC002).

语种：英文

外文关键词：Pterocarpus santalinus and Pterocarpus tinctorius; MALDI-TOF-MSI; chemical fingerprint; species-level timber identification; wood anatomy

摘要：Profiling the spatial distributions and tissue changes of characteristic compounds with interspecific differences is critical to elucidate the complex species identification during tree species traceability, wood anti-counterfeiting verification and timber trade control. In this research, in order to visualize the spatial position of characteristic compounds in two species with similar morphology (Pterocarpus santalinus and Pterocarpus tinctorius), a high coverage MALDI-TOF-MS imaging method was used to found the mass spectra fingerprints of different wood species. 2-Mercaptobenzothiazole matrix was used to spray wood tissue section to enhance the detection effect of metabolic molecules, and the mass spectrometry imaging data were obtained. Based on this technology, the spatial location of fifteen potential chemical markers with remarkable interspecific differences in 2 Pterocarpus timber species were successfully obtained. Distinct chemical signatures obtained from this method can promote rapid identification at the wood species level. Thus, matrix-assisted laser desorption/time-of-flight/ionization mass spectrometry imaging (MALDI-TOF-MSI) provides a spatial-resolved way for traditional wood morphological classification and breaking through the limitations of traditional wood identification technology.