文献类型：期刊文献

中文题名：灰背杜鹃菌根真菌的分离与鉴定及DGGE的应用

英文题名：Isolation and identification of mycorrhizal fungi from Rhododendron hippophaeoides and application of DGGE

作者：刘振华[1,2] 李潞滨[2] 童方平[1] 姚娜[2]

第一作者：刘振华

机构：[1]湖南省林业科学院;[2]中国林业科学研究院林业研究所

年份：2012

卷号：39

期号：4

起止页码：16-20

中文期刊名：湖南林业科技

外文期刊名：Hunan Forestry Science & Technology

基金：中央级公益性科研院所专项资金项目(RIF2010-16)

语种：中文

中文关键词：灰背杜鹃;菌根真菌;DGGE;分离;鉴定

外文关键词：Rhododendron hippophaeoides; mycorrhizal fungi ; DGGE; isolation; identification

分类号：S685.21

摘要：应用传统的培养方法分离菌根真菌,应用ITS rDNA分子生物学手段,进行了菌株的分类鉴定,并结合DGGE(变性梯度凝胶电泳)技术对菌根真菌进行检测。传统培养得到了2种与杜鹃花菌根真菌亲缘关系十分密切的真菌,分别为Phialocephala fortinii和Epacris pulchella root associated fungus。DGGE(变性梯度凝胶电泳)技术检测到1种菌根真菌为Rhizoscyphus ericae。
In order to understand the mycorrhizal fungi diversity of Rhododendron hippophaeoides, the mycorrhizal fungi was isolated from Rhododendron hippophaeoides, and then it was identified by both traditional classification method of morphology and rDNA ITS sequence analysis of molecular biology techniques. Two kinds of mycorrhizal strains were isolated by the traditional culture method, re- spectively： Phialocephala fortinii and Epacris pulchena root associated fungus. On the other hand, Rhizoscsphus er/cae was isolated and identified by Denaturing Gradient Gel Electrephoresis （DGGE） technique.