文献类型：期刊文献

英文题名：Growth-regulating factor 15 is required for leaf size control in Populus

作者：Zhou, Houjun[1,2] Song, Xueqin[1,2,3] Wei, Kaili[1,2] Zhao, Yanqiu[1,2] Jiang, Cheng[1,2] Wang, Jinnan[1,2] Tang, Fang[1,2,3] Lu, Mengzhu[1,2,3]

通信作者：Lu, MZ[1];Lu, MZ[2];Lu, MZ[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat, State Forestry Adm, Res Inst Forestry, Beijing 100091, Peoples R China;[3]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Jiangsu, Peoples R China

年份：2019

卷号：39

期号：3

起止页码：381-390

外文期刊名：TREE PHYSIOLOGY

收录：;Scopus(收录号：2-s2.0-85064807515);WOS:【SCI-EXPANDED(收录号:WOS:000467968800005)】；

基金：This work was supported by the National Natural Science Foundation of China (31570676), the Basic Research Fund of Research Institute of Forestry, Chinese Academy of Forestry (RIF-2013-07 and RIF-2014-08).

语种：英文

外文关键词：cell expansion; cell proliferation; GRF15; growth-regulating factor; leaf size; Populus

摘要：Growth-regulating factors (GRFs) are involved in various developmental events, particularly leaf development. However, the functions of GRFs in woody plants remain elusive. In this study, functional characterization of GRF15 in Populus was performed. Most GRFs are preferentially expressed in young leaves. As GRF15 was expressed at the highest level and with highest ratio in Populus species with large leaves, this gene was investigated through transgenic analyses. Promotor--glucuronidase analysis revealed expression of GRF15 at the leaf expansion zone. Additionally, GRF15 was found to be localized in the nucleus and regulated by miR396. Leaf size and palisade cell size were significantly increased and decreased in GRF15-overexpressing and dominant repression lines, respectively. Consistently, expression of EXPA11a, a homolog of cell-expansion marker EXPA11 in Arabidopsis, was strongly upregulated and downregulated in the GRF15-overexpressing and dominant repression lines, respectively, which was further manifested by activation of EXPA11a by GRF15 in transactivation assays. Therefore, GRF15 is required for leaf size control and primarily modulates cell expansion during leaf development in Populus.