文献类型：期刊文献

英文题名：Selection of reference genes for quantitative real-time PCR in Casuarina equisetifolia under salt stress

作者：Fan, C.[1] Qiu, Z.[1] Zeng, B.[1] Liu, Y.[1] Li, X.[1] Guo, G.[1]

第一作者：Fan, C.

通信作者：Fan, C[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China

年份：2017

卷号：61

期号：3

起止页码：463-472

外文期刊名：BIOLOGIA PLANTARUM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000405279000007)】；

基金：We are grateful to the Ministry of Science and Technology of China (2013AA102705) and the Fundamental Research Funds for central public welfare research institutes (RITFYWZX201304) for financial support. We are also grateful to Dr. Zhang Yong (the Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, China) for providing experimental material.

语种：英文

外文关键词：ACT; EF1a; DNAJ; GAPDH; U2; UBC

摘要：Real time quantitative PCR (qPCR) is widely used in gene expression analysis for its accuracy and sensitivity. Reference genes serving as endogenous controls are necessary for gene normalization. In order to select an appropriate reference gene to normalize gene expression in Casuarina equisetifolia under salt stress, 10 potential reference genes were evaluated using real time qPCR in the leaves and roots of plants grown under different NaCl concentrations and treatment durations. GeNorm, NormFinder, and BestKeeper analyses reveal that elongation factor 1-alpha (EF1 alpha) and ubiquitin-conjugating enzyme E2 (UBC) were the most appropriate reference genes for real time qPCR under salt stress. However, beta-tubulin (beta TUB) and actin 7, which were widely used as reference genes in other plant species, were not always stably expressed. The combination of EF1 alpha, UBC, uncharacterized protein 2, DNAJ homolog subfamily A member 2, and glyceraldehyde-3-phosphate dehydrogenase should be ideal reference genes for normalizing gene expression data in all samples under salt stress. It indicates the need for reference gene selection for normalizing gene expression in C. equisetifolia. In addition, the suitability of reference genes selected was confirmed by validating the expression of WRKY29-like and expansin-like B1. The results enable analysis of salt response mechanism and gene expression in C. equisetifolia.