文献类型：期刊文献

英文题名：Genome-Wide Identification, Classification, and Expression Analysis of 14-3-3 Gene Family in Populus

作者：Tian, Fengxia[1,2] Wang, Tan[1,2] Xie, Yuli[1,2] Zhang, Jin[2] Hu, Jianjun[2]

第一作者：Tian, Fengxia

通信作者：Zhang, J[1]

机构：[1]Nanyang Normal Univ, Coll Life Sci & Technol, Nanyang, Henan, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing, Peoples R China

年份：2015

卷号：10

期号：4

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000352845100153)】；

基金：This work was supported by National Nonprofit Institute Research Grant of CAF [TGB2013009] and [CAFYBB2014ZX001-4] to JJH and the China Postdoctoral Science Foundation [2014M550104] to JZ. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：Background In plants, 14-3-3 proteins are encoded by a large multigene family and are involved in signaling pathways to regulate plant development and protection from stress. Although twelve Populus 14-3-3s were identified based on the Populus trichocarpa genome V1.1 in a previous study, no systematic analysis including genome organization, gene structure, duplication relationship, evolutionary analysis and expression compendium has been conducted in Populus based on the latest P. trichocarpa genome V3.0. Principal Findings Here, a comprehensive analysis of Populus 14-3-3 family is presented. Two new 14-3-3 genes were identified based on the latest P. trichocarpa genome. In P. trichocarpa, fourteen 14-3-3 genes were grouped into epsilon and non-epsilon group. Exon-intron organizations of Populus 14-3-3s are highly conserved within the same group. Genomic organization analysis indicated that purifying selection plays a pivotal role in the retention and maintenance of Populus 14-3-3 family. Protein conformational analysis indicated that Populus 14-3-3 consists of a bundle of nine a-helices (alpha 1-alpha 9); the first four are essential for formation of the dimer, while alpha 3, alpha 5, alpha 7, and alpha 9 form a conserved peptide-binding groove. In addition, alpha 1, alpha 3, alpha 5, alpha 7, and alpha 9 were evolving at a lower rate, while alpha 2, alpha 4, and alpha 6 were evolving at a relatively faster rate. Microarray analyses showed that most Populus 14-3-3s are differentially expressed across tissues and upon exposure to various stresses. Conclusions The gene structures and their coding protein structures of Populus 14-3-3s are highly conserved among group members, suggesting that members of the same group might also have conserved functions. Microarray and qRT-PCR analyses showed that most Populus 14-3-3s were differentially expressed in various tissues and were induced by various stresses. Our investigation provided a better understanding of the complexity of the 14-3-3 gene family in poplars.