文献类型：期刊文献

英文题名：SALT STRESS RESISTANCE IN SMCP-TRANSGENIC ARABIDOPSIS THALIANA AS REVEALED BY TRANSCRIPTOME ANALYSIS

作者：Li, He[1,2,3] Zheng, Liu[1,2] Wang, Ying[1,2] Hu, Xianqi[3] Lu, Zhuchou[1,2] Fan, Huijin[1,2] Zhang, Zhuang[1,2] Li, Yixiao[4] Zhuo, Renying[1,2] Qiu, Wenmin[1,2]

第一作者：Li, He

通信作者：Zhuo, RY[1];Qiu, WM[1];Zhuo, RY[2];Qiu, WM[2]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Xiangshan Rd, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[3]Yunnan Agr Univ, Coll Plant Protect, Kunming 650201, Yunnan, Peoples R China;[4]Prod Promot Ctr Hangzhou, Hangzhou 310012, Peoples R China

年份：2020

卷号：52

期号：3

起止页码：735-752

外文期刊名：PAKISTAN JOURNAL OF BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000522404600001)】；

基金：This work was supported by a grant from National Transformation Science and Technology Program (2018ZX08020002-005-003), the National Natural Science Foundation of China (No. 31370600), and the Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding (No. 2016C02056-1).

语种：英文

外文关键词：SmCP; Salt stress; Transcriptome

摘要：Adaptation to environmental changes is crucial for the viability of all organisms. In plants, cysteine proteases (CP) are vital proteolytic enzymes response to complex and volatile environmental factors. Previously, over-expression of a CP gene isolated from Salix matsudana (SmCP) was shown to improve the salt stress tolerance of Arabidopsis thaliana. However, the molecular mechanisms that underlie the enhanced salt stress tolerance of these over-expression lines remain uncharacterized. In this study, the transcriptome of transgenic Arabidopsis SmCP over-expression lines and wild type (WT) control (CT) plants was analyzed by RNA sequencing to identify genes associated with salt tolerance. The abundance level of selected differentially expressed genes was validated by quantitative real-time PCR analysis. The SmCP-transgenic line showed many transcriptomic changes under salt-stress conditions, including genes associated with alterations in the antioxidant environment and ion-transport capacity. Elucidation of the mechanism of salt stress resistance is important for utilization of SmCP for genetic improvement of commercial crops for tolerance to saline soil.