文献类型：期刊文献

英文题名：Expression Patterns of MYB (V-myb Myeloblastosis Viral Oncogene Homolog) Gene Family in Resistant and Susceptible Tung Trees Responding to Fusarium Wilt Disease

作者：Wang, Xue[1,2] Zhang, Qiyan[1,2] Gao, Ming[1,2] Wu, Liwen[1,2] Wang, Yangdong[1,2] Chen, Yicun[1,2]

通信作者：Chen, YC[1];Chen, YC[2]

机构：[1]Chinese Acad Forestry, State Key Laboratoty Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2019

卷号：10

期号：2

外文期刊名：FORESTS

收录：;EI(收录号：20191506749497);Scopus(收录号：2-s2.0-85063878462);WOS:【SCI-EXPANDED(收录号:WOS:000460744000117)】；

基金：The work has been financially supported by science and Technology Major Program on Agricultural New Variety Breeding of Zhejiang, China (no. 2016C02056); "Ten thousand people plan" Science and Technology Innovation Leading Talent of Zhejiang, China (no. 2018R52006); National Key R&D Program of China (no. 2017YFD0600704) awarded to Y.-C.C.

语种：英文

外文关键词：MYB; transcription factors; Fusarium wilt disease; tung oil tree; expression pattern

摘要：Vernicia fordii (tung oil tree) is famous in the world for its production of tung oil. Unfortunately, it was infected by the soil-borne fungus Fusarium oxysporum f. sp. fordii 1 (Fof-1) and suffered serious wilt disease. Conversely, its sister species V. montana is highly resistant to Fof-1. The MYB (v-myb myeloblastosis viral oncogene homolog) transcription factors were activated during the pathogen Fof-1 infection according to our previous comparative transcriptomic results. Depending on whether the sequence has a complete MYB-DNA-binding domain, a total of 75 VfMYB and 77 VmMYB genes were identified in susceptible V. fordii and resistant V. montana, respectively. In addition, we detected 49 pairs of one-to-one orthologous Vf/VmMYB genes with the reciprocal-best BLAST-hits (RBH)method. In order to investigate the expression modes and the internal network of MYB transcription factors in the two species responding to Fusarium wilt disease, the expressions of Vf/VmMYBs were then investigated and we found that most orthologous Vf/VmMYB genes exhibited similar expression patterns during the Fof-1 infection. However, four pairs of Vf/VmMYB genes, annotated as unknown proteins and mediator of root architecture, demonstrated absolute opposite expression patterns in the two Vernicia species responding to Fof-1. The interaction network of VmMYB genes were further constructed using weighted gene co-expression network analysis (WGCNA) method and four hub genes showing extremely high interaction with the other 1157 genes were identified. RT-qPCR result verified the opposite expression pattern of the hub gene VmMYB011 and VmMYB041 in two Vernicia species. In summary, co-expression network of the Vf/VmMYBs and significantly opposite related pairs of genes in resistant and susceptible Vernicia species provided knowledge for understanding the molecular basis of Vernicia responding to Fusarium wilt disease.