文献类型：期刊文献

英文题名：Rosmarinic acid-chondroitin sulfate nanoconjugate for targeted treatment of ulcerative colitis

作者：Long, Miaomiao[1,2] Li, Jie[3] Yang, Meiyang[3] Chen, Weijun[3] Qiu, Lipeng[3] Cheng, Xian[1] Bi, Liangwu[1]

第一作者：Long, Miaomiao

通信作者：Cheng, X[1];Bi, LW[1];Yang, MY[2]

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Nanjing 210042, Peoples R China;[2]Wuxi Higher Hlth Vocat Technol Sch, Dept Pharm, Wuxi 214028, Peoples R China;[3]Jiangnan Univ, Sch Life Sci & Hlth Engn, Wuxi 214122, Peoples R China

年份：2025

卷号：306

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20251017990109);Scopus(收录号：2-s2.0-85219371254);WOS:【SCI-EXPANDED(收录号:WOS:001441603500001)】；

基金：This work was supported by the National Key Research and Devel-opment Program of China (2021YFC2103100) , Research Project of Wuxi Health Commission (M202323) and National Natural Science Founda-tion of China (32201512) .

语种：英文

外文关键词：Rosmarinic acid; Chondroitin sulfate; Ulcerative colitis

摘要：Rosmarinic acid (RA) is an attractive candidate for ulcerative colitis (UC) application due to its bioactive properties, including antioxidant and anti-inflammatory functions, however, the poor water solubility and on-targeting hamper its therapeutic outcome. Therefore, this work reported the synthesis and preparation of novel water-soluble rosmarinic acid-chondroitin sulfate A (RA-CSA) nanoconjugate, which was used for the treatment of UC in dextran sulfate sodium (DSS)-induced acute colitis mouse model. RA was functionalized with CSA as confirmed by FTIR and H-1 NMR, and self-assembled to form nanoassemblies with a diameter of 247.3 +/- 2.99 nm. RA-CSA nanoassemblies exhibited radical scavenging and antioxidant capacity. RA-CSA remarkably inhibited lipopolysaccharide-induced nitric oxide and TNF-alpha production in RAW 264.7 cells without cytotoxicity, whose inhibition rate was <5 % at 200 mu g mL(-1). Oral administration of RA-CSA nanoassemblies significantly attenuated colonic inflammation compared to the parent RA, as evidenced by significantly reduced the shortening of colon length (4.20 +/- 0.15 cm), body weight loss, and colonic inflammatory damage in DSS-induced colitis mice. In addition, RA-CSA nanoassemblies suppressed the expression and production of typical pro-inflammatory cytokines of ulcerative colitis. These results suggest that RA-CSA nanoassemblies deserve further consideration as a potential therapeutic drug for the treatment of UC.