文献类型：期刊文献

中文题名：盐胁迫对桑树幼苗光合生理及叶绿素荧光特性的影响

英文题名：Effects of Salinity on Photosynthetic Physiology and Chlorophyll Fluorescence Characteristics of Mulberry(Morus alba) Seedlings

作者：柯裕州[1] 周金星[1] 卢楠[2] 张旭东[1] 孙启祥[1]

第一作者：柯裕州

机构：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091;[2]北京林业大学生物科学与技术学院,北京100083

年份：2009

卷号：22

期号：2

起止页码：200-206

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家“十一五”科技支撑项目“长江中下游低山丘陵生态退化区植被恢复技术实验示范”(2006BAD03A16)

语种：中文

中文关键词：桑树;盐胁迫;光合特性;叶绿素荧光特性

外文关键词：mulberry （ Morus alba） ; salt stress ; photosynthetic physiology ; chlorophyll fluorescence characteristics

分类号：S718.43

摘要：试验以黄河流域和西北地区常用的桑树砧木-1年生实生桑树幼苗为试验材料,采用盆栽加盐的方式模拟盐胁迫环境,通过设置1、3、5、7 g.kg-1等4个盐分梯度,以不加NaCl(0 g.kg-1)为对照(CK),研究NaCl胁迫对桑树幼苗光合作用和叶绿素荧光特性的影响。结果表明:1 g.kg-1NaCl处理对桑树幼苗叶片的净光合速率(Pn)没有明显影响;而3、5、7 g.kg-1等NaCl处理则对Pn具有明显的抑制作用,5、7g.kg-1等的NaCl处理的影响显著,造成桑树幼苗Pn急剧降低,部分植株受害严重,甚至死亡。此外,1、3 g.kg-1等NaCl处理对桑树幼苗的气孔导度(Gs)、蒸腾速率(Tr)、水分利用率(WUE)和胞间CO2浓度(Ci)没有明显影响,而5、7 g.kg-1等NaCl处理则显著提高了桑树幼苗的Ci,降低了Gs、Tr和WUE。盐胁迫对桑树幼苗叶片的叶绿素荧光参数具有一定的影响。随NaCl处理浓度增大,初始荧光(Fo)呈下降的趋势,而非光化学荧光淬灭系数(NPQ)则先升后降,但处理之间Fo和NPQ变化差异不明显;1、3 g.kg-1等NaCl处理对桑树幼苗的最大荧光(Fm)、最大光化学效率(Fv/Fm)、潜在光化学效率(Fv/Fo)和PSⅡ有效光化学量子效率(ΦPSⅡ)没有明显影响,而5、7 g.kg-1等NaCl处理则显著降低其Fm、Fv/Fm、Fv/Fo和ΦPSⅡ。
The pot experiments on effects of salt stress on photosynthetic physiology and chlorophyll fluorescence characteristics of 1-year-old mulberry （Morus alba） seedlings were conducted with a common stock of mulberry- Shishengsang which adapted to the Yellow River valley and northwestern areas. The seedlings were treated with NaCI concentrations of 1,3, 5 and 7g·kg^-1, and with fresh water as the control. By using LI-COR 6400 portable photosynthesis system and PAM-2100 portable pulse-modulated chlorophyll fluorescence system, the net photosynthetic rate （Pn） , stomatal conductance （ Gs ） and other indexes were measured on the 1 st, 2nd,3rd, that the salinity in NaCl concentration of 1 g·kg^-1 , intercellular CO2 concentrations （ Ci）, transpiration rate （Tr） 7th and 21 th day after salt stress treatments. The results showed had no significant impact on Pn of mulberry seedlings, but it significantly inhibited Pn under treatment with NaCI concentration of 3, 5 and 7 g·kg^-1 , especially, the effects of salt in NaCl concentration of 5 and 7g·kg^-1 on it were greater. Moreover, Pn gradually decreased as NaCl concentration increased. In addition, the salt had no significant effects on Gs, Ci, Tr and WUE under treatments with NaCl concentration of 1 and 3g·kg^-1, however Gs, Tr and WUE significantly decreased but Ci significantly increased when the mulberry seedlings were treated with NaCl concentration of 5 and 7g·kg^-1. Moreover, the salinity had impact on the kinetic parameters of chlorophyll fluorescence of mulberry seedling leaves. During the experiments, the original fluorescence （Fo） showed a declining trend, but non-photochemical quenching of fluorescence （NPQ） were at first increased and then decreased with the increment of NaCl concentration, however, the differences of Fo and NPQ under various salt stress treatments were not significant. However, salinity didn＇t significantly affect the maximal fluorescence（ Fm）, original light energy transforming efficiency （Fv / Fm）, PSⅡ latent activity （ Fv / Fo） and quantum yield of PS Ⅱ electron transport （ ΦPS Ⅱ ） of mulberry seedling leaves under treatments with NaCl concentrations of 1 and 3 g·kg^-1, but Fm, Fv / Fm, Fv / Fo and ΦPS Ⅱ evidently declined when treated with NaCl concentrations of 5 and 7 g·kg^-1. It denoted that NaCl critical concentration of mulberry seedlings of 1 year old was between 3 and 5g·kg^-1.