文献类型：期刊文献

英文题名：Effects of tung oilseed FAD2 and DGAT2 genes on unsaturated fatty acid accumulation in Rhodotorula glutinis and Arabidopsis thaliana

作者：Chen, Yicun[1,2] Cui, Qinqin[1,2] Xu, Yongjie[3] Yang, Susu[1,2] Gao, Ming[1,2] Wang, Yangdong[1,2]

第一作者：陈益存

通信作者：Wang, YD[1]|[a0005f34f1ecf07bb8f24]汪阳东;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[3]Hubei Forestry Acad, Wuhan 430075, Hubei, Peoples R China

年份：2015

卷号：290

期号：4

起止页码：1605-1613

外文期刊名：MOLECULAR GENETICS AND GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000358246000033)】；

基金：The work has been financially supported by the Special Fund for Forestry-scientific Research in the Public Interest (201304102) and the lecture and study program for outstanding scholars from home and abroad (CAFYBB2011007).

语种：英文

外文关键词：FAD2; DGAT2; Unsaturated fatty acid; Transgenic

摘要：Genetic engineering to produce valuable lipids containing unsaturated fatty acids (UFAs) holds great promise for food and industrial applications. Efforts to genetically modify plants to produce desirable UFAs with single enzymes, however, have had modest success. The key enzymes fatty acid desaturase (FAD) and diacylglycerol acyltransferase (DGAT) are responsible for UFA biosynthesis (a push process) and assembling fatty acids into lipids (a pull process) in plants, respectively. To examine their roles in UFA accumulation, VfFAD2 and VfDGAT2 genes cloned from Vernicia fordii (tung tree) oilseeds were conjugated and transformed into Rhodotorula glutinis and Arabidopsis thaliana via Agrobacterium tumefaciens. Real-time quantitative PCR revealed variable gene expression levels in the transformants, with a much higher level of VfDGAT2 than VfFAD2. The relationship between VfFAD2 expression and linoleic acid (C18:2) increases in R. glutinis (R (2) = 0.98) and A. thaliana (R (2) = 0.857) transformants was statistically linear. The VfDGAT2 expression level was statistically correlated with increased total fatty acid content in R. glutinis (R (2) = 0.962) and A. thaliana (R (2) = 0.8157) transformants. With a similar expression level between single- and two-gene transformants, VfFAD2-VfDGAT2 co-transformants showed a higher linolenic acid (C18:3) yield in R. glutinis (174.36 % increase) and A. thaliana (14.61 % increase), and eicosatrienoic acid (C20:3) was enriched (17.10 % increase) in A. thaliana. Our data suggest that VfFAD2-VfDGAT2 had a synergistic effect on UFA metabolism in R. glutinis, and to a lesser extent, A. thaliana. These results show promise for further genetic engineering of plant lipids to produce desirable UFAs.