文献类型：期刊文献

英文题名：Improved genetic transformation of Jatropha curcas using phosphomannose isomerase as biosafe selectable marker

作者：Chen, Jienan[1,3] Liu, Bobin[1,2] Lu, Mengzhu[1,2] Zhao, Shutang[2] Zhang, Lin[1]

第一作者：Chen, Jienan

通信作者：Chen, Jienan

机构：[1] Ministry of Forestry Bioethanol Research Center, Institute of Biological and Environmental Science and Technology, Central South University of Forestry and Technology, Changsha, China; [2] State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China; [3] Transpoints Inc., P.O. Box 141742, Gainesville, FL, United States

年份：2015

卷号：9

期号：1

起止页码：9-15

外文期刊名：Journal of Biobased Materials and Bioenergy

收录：EI(收录号：20152000854924);Scopus(收录号：2-s2.0-84929151207)

语种：英文

外文关键词：Polymerase chain reaction - Plants (botany)

摘要：An Agrobacteriuim-mediated Jatropha curcas transformation procedure has been optimized around high frequency leaf disc regeneration using phosphomannose isomerase (pmi) gene as biosafe selectable marker. A mannose to sucrose ratio of (20 g/10 g)·L-1 was found to be optimal as selection pressure. Cephalothin as a bacteriostat was optimized at 500 mg·L-1 for inhibition of Agrobacteriuim overgrowth without destructive effect on callus induction. The effect of acetosyringone concentration, inoculation duration, and co-cultivation time was evaluated and found to be most desirable at 0.02 mM, 10 min and 2 days, respectively. Sonication treatment had a suppressing effect on resistant shoots induction. The transformation efficiency with mannose selection was twice of that with kanamycin selection. PCR amplification identified 6 positive plants from 12 independent mannose-resistant plants. This efficient and biosafe transformation system is of significance to facilitating the genetic improvement of J. curcas as a promising biodiesel plant and also to alleviating public concerns on the wide use of transgenic energy plants. Copyright ? 2015 American Scientific Publishers All rights reserved.