文献类型：期刊文献

英文题名：Pollen competition and paternal contribution during artificially controlled pollination of black locust (Robinia pseudoacacia l.) without castration

作者：Sun, Yuhan[1] Hu, Ruiyang[2] Dong, Li[1] Li, Xiuyu[1] Zhang, Zijie[1] Guo, Qi[1,3] Cao, Sen[1] Li, Jiankang[1] Han, Peiyao[1] Han, Chao[1] Uddin, Saleem[1] Long, Cui[1] Fan, Yingming[1] Li, Yun[1]

第一作者：Sun, Yuhan

机构：[1] Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; [2] Experimental Center of Forestry in North China, Chinese Academy of Forestry, National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain in Beijing, Beijing, 102300, China; [3] College of Agriculture, Henan University of Science and Technology, Luoyang, 471023, China

年份：2021

卷号：12

期号：10

外文期刊名：Forests

收录：EI(收录号：20214411102695);Scopus(收录号：2-s2.0-85118200258)

语种：英文

外文关键词：Artificial controlled pollination; Honeybee; Hybridization breeding; Outcrossing rate; Pollen competition; Robinia pseudoacacia L; Without castration

摘要：(1) Background: Considering the serious damage caused by castration and the extremely high outcrossing rate in nature, we hypothesized that artificial controlled pollination of black locust without castration could be conducted for hybridization breeding. (2) Methods: The study conducted controlled pollination on 20 mating combinations of black locust without castration using a single or mixed male parent. Offspring of different developmental stages and the leaves of parents were collected to extract DNA and perform paternity analysis using SSR molecular markers. (3) Results: The contribution rate of each male parent differed according to developmental stage after pollination using different pollens mixed in equal proportions. There were significant correlations between the genetic similarity between each male parent and female parent and contribution rate of each male parent at three different developmental stages after pollination. (4) Conclusions: The composition of offspring pollen donors showed no bias toward selfing or outcrossing when artificially pollinated without castration. Hybrid breeding of black locust by artificially controlled pollination without castration may not be feasible, given that our manual method resulted in a large number of abortive and abnormal offspring. Introduction of honeybees in a limited space to conduct controlled pollination of black locust for hybrid breeding may be feasible. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.