文献类型：期刊文献

中文题名：血红色钉菇菌塘土壤微生物群落多样性

英文题名：Microbial community diversity of mushroom-producing soil of Chroogomphus rutilus

作者：王兴红[1] 边禄森[1] 刘慧慧[1] 马庆华[1] 陈淋[1] 苗朔[1]

第一作者：王兴红

机构：[1]中国林业科学研究院华北林业实验中心北京九龙山暖温带国家森林长期科研基地,北京102300

年份：2025

卷号：44

期号：6

起止页码：15-29

中文期刊名：菌物学报

外文期刊名：Mycosystema

收录：;北大核心:【北大核心2023】；

基金：中国林业科学研究院中央级公益性科研院所基本科研业务费专项资金(CAFYBB2022ZC006)。

语种：中文

中文关键词：血红色钉菇;菌塘土壤;高通量测序;微生物群落多样性

外文关键词：Chroogomphus rutilus;mushroom-producing soil;high-throughput sequencing;microbial community diversity

分类号：S646

摘要：菌塘是菌根食用菌形成子实体的重要场所,菌塘中存在可以促进菌根形成的微生物,因此对菌塘土壤微生物群落的系统研究和深入分析对于了解食用菌菌根形成至关重要。为明确血红色钉菇菌塘土壤中微生物群落组成和多样性,本研究对血红色钉菇菌塘与非菌塘土壤样品进行扩增子测序,结果显示非菌塘土壤细菌和真菌的Chao 1、Observed species、Shannon、Simpson、Pielou指数显著大于菌塘土壤(P<0.05);菌塘与非菌塘土壤细菌优势门均为变形菌门Proteobacteria,在菌塘土中,变形菌门的相对丰度大于其在非菌塘土中的相对丰度;菌塘与非菌塘土壤优势真菌门为担子菌门Basidiomycota,在菌塘土中,子囊菌门Ascomycota的相对丰度高于非菌塘土;罗兹菌门Rozellomycota的相对丰度是非菌塘土相对丰度的2.94倍;菌塘与非菌塘土壤真菌优势属为红菇属Russula和乳牛肝菌属Suillus,在菌塘中,Leptodontidium属的相对丰度为非菌塘中的1.27倍,青霉属Penicillium的相对丰度为非菌塘中相对丰度的4.47倍,乳牛肝菌属的相对丰度为非菌塘相对丰度的5.72倍,树粉孢属Oidiodendron相对丰度为非菌塘中相对丰度的6.31倍,篮状菌属Talaromyces的相对丰度为非菌塘中相对丰度的18.14倍,其中色钉菇属Chroogomphus和蜡壳耳属Sebacina只存在于菌塘土壤中。LEfSe分析揭示相对于非菌塘土壤,菌塘土壤中共获得26个不同分类水平(1个门、1个纲、4个目、6个科、9个属、5个种)的真菌和20个不同分类水平(2个门、3个纲、3个目、6个科、6个属)的细菌指示类群。共线性网络分析表明菌塘和非菌塘土壤真菌和细菌群落的节点数和边数差异不显著,菌塘土壤中血红色钉菇与细菌ASV20(变形菌门未培养菌)和真菌ASV22(绒毛菌属Tomentella)的丰度呈正相关,且相关性较高。研究结果将为血红色钉菇的人工驯化栽培提供理论基础。
Mushroom-producing soil is the important place for the fruiting body formation of mycorrhizal edible fungi.Microorganisms that can promote the mycorrhizal formation exist in the mushroom-producing soil,so the study of microbial communities of the mushroom-producing soil is crucial for understanding the mycorrhizal formation.In this study,the composition of microbial communities and diversity of the mushroom-producing soil of Chroogomphus rutilus and non-mushroom-producing soil were clarified by use of amplicon sequencing.The results showed that the Chao 1,Observed species,Shannon,Simpson and Pielou indices of bacteria and fungi in the non-mushroom-producing soil were significantly higher than those in the mushroomproducing soil(P<0.05).The dominant phyla in both the mushroom-producing and non-mushroomproducing soil are Proteobacteria and Basidiomycota.In the mushroom-producing soil,the relative abundance of Proteobacteria,Ascomycota and Rozellomycota is greater than that in the non-mushroom-producing soil,among them the relative abundance of Rozellomycota is 2.94 times higher than that in the non-mushroom-producing soil.The dominant genera in the mushroomproducing and non-mushroom-producing soil are Russula and Suillus.In the mushroomproducing soil,the relative abundance of Leptodontidium is 1.27 times higher than that in the non-mushroom-producing soil,the relative abundance of Penicillium is 4.47 times higher than that in the non-mushroom-producing soil,the relative abundance of Suillus is 5.72 times higher than that in the non mushroom-producing soil,the relative abundance of Oidiodendron is 6.31 times higher than that in the non-mushroom-producing soil,the relative abundance of Talaromyces is 18.14 times higher than that in the non-mushroom-producing soil,and the genus Chroogomphus and Sebacina only exists in the mushroom-producing soil.LEfSe analysis showed that 26 different taxonomic levels of fungi(1 phylum,1 class,4 orders,6 families,9 genera,5 species)and 20 different taxonomic levels of bacterial indicator groups(2 phyla,3 classes,3 orders,6 families,6 genera)were obtained in the mushroom-producing soil.The co-occurrence network analysis showed that there was no significant difference in the number of nodes and edges of fungal and bacterial communities between the mushroom-producing and non-mushroom-producing soil.Chroogomphus rutilus was positively correlated with the abundance of unidentified bacterium ASV20(uncultured Proteobacteria)and that of unidentified fungus ASV22(Tomentella),and the correlation was high.The results provide reference for the artificial domestication and cultivation of C.rutilus.