文献类型：期刊文献

英文题名：Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Salix matsudana under different abiotic stresses

作者：Zhang, Yunxing[1,2,3] Han, Xiaojiao[1,2] Chen, Shuangshuang[1,2] Zheng, Liu[1,2] He, Xuelian[1,2] Liu, Mingying[1,2] Qiao, Guirong[1,2] Wang, Yang[4] Zhuo, Renying[1,2]

第一作者：Zhang, Yunxing

通信作者：Zhuo, RY[1];Zhuo, RY[2];Wang, Y[3]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Zhejiang, Peoples R China;[3]Henan Polytech Univ, Sch Architectural & Artist Design, Jiaozuo 454000, Henan, Peoples R China;[4]Yunnan Agr Univ, Coll Plant Protect, Kunming 650201, Yunnan, Peoples R China

年份：2017

卷号：7

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000392658900001)】；

基金：This work was supported by the National Nonprofit Institute Research Grant of Chinese Academy of Forestry (CAFYBB2017ZY007, No. TGB2013008, No. RISF2014010), the National High Technology Research and Development Program of China (No. 2013AA102701-3), the Science and Technology Department of Zhejiang Province (No. 2016C32027), and a Nonprofit Research Grant of Zhejiang Province (No. 2016C32G3030016).

语种：英文

摘要：Salix matsudana is a deciduous, rapidly growing willow species commonly cultivated in China, which can tolerate drought, salt, and heavy metal stress conditions. Selection of suitable reference genes for quantitative real-time PCR is important for normalizing the expression of the key genes associated with various stresses. To validate suitable reference genes, we selected 11 candidate reference genes (five traditional housekeeping genes and six novel genes) and analyzed their expression stability in various samples, including different tissues and under different abiotic stress treatments. The expression of these genes was determined using five programs-geNorm, NormFinder, BestKeeper, Delta Ct, and RefFinder. The results showed that alpha-TUB2 (alpha-tubulin 2) and DnaJ (chaperone protein DnaJ 49) were the most stable reference genes across all the tested samples. We measured the expression profiles of the defense response gene SmCAT (catalase) using the two most stable and one least stable reference genes in all samples of S. matsudana. The relative quantification of SmCAT varied greatly according to the different reference genes. We propose that alpha-TUB2 and DnaJ should be the preferred reference genes for normalization and quantification of transcript levels in future gene expression studies in willow species under various abiotic stress conditions.