文献类型：期刊文献

中文题名：土地利用变化对土壤真菌群落结构的影响

英文题名：The impact of land use changes on soil fungal community and structure

作者：张于光[1] 张小全[1] 曲良建[1] 肖烨[2]

第一作者：张于光

机构：[1]中国林业科学研究院森林生态环境与保护研究所;[2]湖南农业大学生物安全科技学院

年份：2007

卷号：27

期号：10

起止页码：4325-4332

中文期刊名：生态学报

外文期刊名：Acta Ecologica Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助项目(40271109)~~

语种：中文

中文关键词：土地利用变化;真菌;群落结构;18S;rDNA;PCR-RFLP

外文关键词：land use changes; fungi; community and structure; 18S rDNA; PCR-RFLP

分类号：Q938;S154.36

摘要：应用PCR-RFLP和测序分析对川西亚高山米亚罗林区不同土地利用类型的土壤真菌18SrDNA基因进行了多样性和系统发育研究，探讨了土地利用变化对土壤真菌群落结构的影响。在20a龄云杉（Picea likiandensis var balfourianan）人工林和菜地两种类型土壤中，共得到238个阳性克隆，限制性内切酶MspⅠ和RsaⅠ进行RFLP分析后得到56个不同的分类操作单元（OTUs），其中20a龄云杉人工林样地获得137个阳性克隆和37个OTUs，而菜地样地获得101个阳性克隆和19个OTUs。在两类样地中具有不同的优势种群，其中20a龄云杉人工林样地有1个明显优势种群，占总克隆数的20．4％；菜地样地有2个明显优势种群，分别占总克隆数的25．7％和21．8％。对14个克隆进行了序列测定，序列的相似性在86％一99％之间，与GenBank数据库中的序列进行比对，与已知序列的相似性在92％～100％之间。系统发育分析表明，所有的18SrDNA基因被分为3个主要的簇，其中20a龄云杉人工林样地的克隆都聚集在第一和第三簇中，而菜地样地的克隆都聚集在第二簇中。结果说明，两类土壤中具有较为丰富的真菌多样性，而土地利用变化引起了土壤真菌群落结构的明显变化。
To understand the impact of land use changes on the composition and structure of fungal communities from Miyaluo county in subalpine forest area of western Sichuan, the molecular diversity of 18S rDNA genes from soil obtained at a 20-year-old spruce plantation （Picea likiandensis var balfourianan） and cropland sites were examined using a PCR-based cloning approach. DNA was directly extracted from the soil microorganisms and amplified the 18S rDNA gene fragment using PCR by the specific primers of EF4f： 5′-GGAAGGG （A/G）TGTATTTATTAG-3′ and Fung-5r： 5′-GTAAA AGTCCTGGTTCCC-3′. For the gene fragment, diverse PCR products were characterized by cloning, restriction fragment length polymorphism （RFLP） analysis and sequencing. A total of 238 clones and 56 operational taxonomic units （OTUs） which were digested by the restriction enzymes MspI and RsaI were obtained from all samples. The 20-year-old spruce plantation and cropland sites were received 137 and 101 clones and 37 and 19 OTUs, respectively. There were different significant dominant groups of clones occurring in both samples and shared 60TUs. 20-year-old spruce plantation and cropland sites had one and two significant dominant groups which account for 20.4%, 25.7% and 21.8% of all clones, respectively. There also were some secondary dominant groups of clones which account for 7.3% -- 8.9% of all clones. Fourteen 18S rDNA clones were sequenced and their nucleotide identity was from 86% to 99%. Compared the known sequences with the deposited in the data bank （www. ncbi. nlm. nih. gov）, their level of nucleotide identity was from 92% to 100%. The phylogenetic tree was constructed by the Clustal W and Mega soft-wares. 14 sequences could be subdivided into 3 clusters in the phylogenetic tree. The clone sequences of 20-year-old spruce plantation site were completely clustered into the first and the third clusters, and the clone sequences of cropland site only distributed in the second cluster. Therefore, both 20-year-old spruce plantation and cropland sites had the high fungal diversity, and land use changes significant influenced the fungal community and structure.