文献类型：期刊文献

中文题名：镉胁迫下多年生黑麦草的光合生理响应

英文题名：Response of photosynthetic physiology of perennial ryegrass(Lolium perenne) to Cd^(2+) stress

作者：刘俊祥[1,2] 孙振元[1,2] 勾萍[3] 钱永强[1,2] 巨关升[1,2] 韩蕾[1,2]

第一作者：刘俊祥

机构：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室;[2]林木遗传育种国家重点实验室中国林业科学研究院林业研究所;[3]沈阳农业大学林学院

年份：2012

卷号：21

期号：3

起止页码：191-197

中文期刊名：草业学报

外文期刊名：Acta Prataculturae Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：国家"863"项目(2011AA100209)资助

语种：中文

中文关键词：镉;胁迫;多年生黑麦草;光合生理;响应

外文关键词：cadmium; stress; Lolium perenne; photosynthetic physiology; response

分类号：S543.604;Q945

摘要：以多年生黑麦草为植物材料,对其进行0(CK),5,10,20mg/L的Cd2+处理6d,结果表明,Cd2+处理显著降低了地上部、地下部的生物量;随着处理浓度的升高,Cd在地上部、地下部的含量显著升高,地上部Cd的分布比例显著升高,地下部Cd的分布比例显著降低,多年生黑麦草对Cd具有超量吸收和转运的能力。在处理的0,0.25,1,3,6d,测定5mg/L Cd2+处理的多年生黑麦草叶片的气体交换参数、光合色素含量和叶绿素荧光参数,结果表明,Cd2+处理未导致光合色素含量的变化,气体交换参数和叶绿素荧光参数均受到显著的影响。处理1d内,净光合速率(Pn)、气孔导度(Gs)和胞间二氧化碳浓度(Ci)具有相同的变化趋势,Pn的下降是由气孔因素导致的。Pn在处理3和6d分别较未处理显著下降了24.4%和23.4%,非气孔因素对Pn的降低起到了主要作用。蒸腾速率(Tr)在处理1d较未处理显著上升了74.1%,这有助于Cd向地上部转运。潜在光化学效率(Fv/F0)在处理的0.25,1,3d时分别较未处理显著上升了14.4%,21.0%和14.4%。最大光化学效率(Fv/Fm)在处理0.25,1,3d分别较未处理显著上升了3.1%,3.2%和3.1%。实际光化学效率(ΦPSⅡ)、相对电子传递效率(rETR)和光化学淬灭系数(qP)在处理6d较未处理显著下降了25.3%,25.3%和21.1%,叶片PSⅡ的光化学反应能力显著降低。非光化学淬灭系数(qN)在处理0.25,1d分别较未处理显著下降了15.6%,41.5%,随着处理时间的延长,qN值显著升高,起到了光保护的作用。非调节性能量耗散[Y(NO)]在处理0.25,1d分别较未处理显著上升了30.8%,37.8%,处理3和6d下降至与未处理无显著差异。调节性能量耗散[Y(NPQ)]在处理1d比未处理显著下降了36.9%,在6d时显著上升了17.0%,减轻了光合机构的伤害程度。
Effects of Cd2＋ on biomass of Lolium perenne were studied,and Cd accumulative characteristics were also elaborated.L.perenne were treated with Cd2＋（0,5,10,20 mg/L） for 6 days.The biomass of aboveground and underground decreased significantly.With the Cd2＋ concentration increasing,the Cd content in L.perenne increased significantly,and the distribution proportion of Cd in underground decreased significantly,contrary in the aboveground.L.perenne had the excessive ability in absorbing and transporting Cd.In order to explore the photosynthetic physiology response of L.perenne to Cd2＋ stress,explaining the biomass change affected by Cd2＋,L.perenne were treated with Cd2＋（5 mg/L） for 0,0.25,1,3,6 days respectively.The photosynthetic pigments contents did not change significantly,whereas the gas exchange parameters and the chlorophyll fluorescence parameters were affected significantly.Within 1 d,the change of Pn,Gs and Ci were consistent,the reduce of net photosynthesis rate was caused by stomatal limitation factors.When the plants were treated 3 and 6 d,Pn significantly decased by 24.4% and 23.4% compared with untreated,the recuce was caused by non-stomatal limitation factors.Tr significantly increased by 74.1% when treated 1 d,this promoted Cd transporting to aboveground.Within 3 d,Fv/F0 significantly increased by 14.4%,21.0%,14.4% compared with untreated,and Fv/Fm significantly increased by 3.1%,3.2%,3.1% respectively.ΦPSⅡ,rETR and qP significantly decreasd by 25.3%,25.3%,21.1% when treated 6 d,the PSⅡ light reaction ability reduced.The qN significantly decreasd by 15.6% and 41.5% when treated 0.25 and 1 d,then significantly increasd,the photoprotective capacity was increased.Compared with untreated,Y（NO） significantly increased by 30.8% and 37.8% when treated 0.25 and 1 d,and decreased significantly when 3 and 6 d.Y（NPQ） significantly decreased by 36.9% when treated 1 d,whereas significantly incerased by 17.0% when treated 6 d,the extent of photosynthetic apparatus damage was reduced.