文献类型：期刊文献

英文题名：A Novel Full-length IgG Recombinant Antibody Highly Specific to Clothianidin and Its Application in Immunochromatographic Assay

作者：Chang, Yunyun[1] Chen, Yang[1] Jiao, Shasha[1] Lu, Xinying[1] Fang, Yihua[1] Liu, Yihua[1,2] Zhao, Ying[1] Zhan, Xiuping[3] Zhu, Guonian[1] Guo, Yirong[1]

第一作者：Chang, Yunyun

通信作者：Liu, YH[1];Guo, YR[1];Liu, YH[2]

机构：[1]Zhejiang Univ, Inst Pesticide & Environm Toxicol, Key Lab Biol Crop Pathogens & Insects Zhejiang Pr, Minist Agr,Key Lab Mol Biol Crop Pathogens & Inse, Hangzhou 310058, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[3]Shanghai Agr Technol Extens Serv Ctr, Shanghai 201103, Peoples R China

年份：2022

卷号：12

期号：4

外文期刊名：BIOSENSORS-BASEL

收录：;EI(收录号：20231213755755);Scopus(收录号：2-s2.0-85128606779);WOS:【SCI-EXPANDED(收录号:WOS:000785110200001)】；

基金：This work was supported by the Shanghai Science and Technology Commission Research Project (grant number 19391902300) and the National Natural Science Foundation of China (grant number 31871994).

语种：英文

外文关键词：clothianidin; heterologous ic-ELISAs; recombinant full-length IgG; non-competitive surface plasmon resonance assay; gold immunochromatographic assay

摘要：The toxicity of clothianidin to non-target organisms has gradually attracted world-wide attention. It is essential to develop reliable methods for the on-site detection of clothianidin residue. In this study, analogue-based heterologous ic-ELISAs were designed to rapidly screen desirable hybridomas, which could be used for the construction of recombinant antibodies (RAbs) against clothianidin. Based on the antibody variable region genes, two full-length IgG RAbs (1F7-RAb and 5C3-RAb) were produced by the mammalian cell expression system. The performance of the two RAbs was characterized and compared by heterologous ic-ELISAs and non-competitive surface plasmon resonance (SPR) assays. Using heterologous ic-ELISAs, the 1F7-RAb exhibited highly specific and sensitive recognition to clothianidin with an IC50 of 4.62 mu g/L whereas the 5C3-RAb could bind to both clothianidin and dinotefuran. The results of the non-competitive SPR assay further verified that the 1F7-RAb had a higher specificity and affinity to clothianidin than the 5C3-RAb. Finally, a gold immunochromatographic assay based on the novel antibody, 1F7-RAb, was developed for rapid detection of clothianidin with high sensitivity (visual detection limit of 2.5 mu g/L), specificity, and good reproducibility, which can be used as an effective supervision tool for clothianidin residue in agricultural and environmental samples.